Liping Xie, MD, John T. Schroeder, PhD, Jacqueline M

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Presentation transcript:

Human IgE+ and IgE− are not equivalent to mouse highly cytokinergic IgE Liping Xie, MD, John T. Schroeder, PhD, Jacqueline M. Langdon, MS, Rebecca S. Sora-Scott, MS, Toshiaki Kawakami, MD, PhD, Susan M. MacDonald, MD Journal of Allergy and Clinical Immunology Volume 121, Issue 4, Pages 1027-1033 (April 2008) DOI: 10.1016/j.jaci.2007.12.1157 Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Effects of mouse HC IgEs on histamine release and ERK1/2 phosphorylation of mouse BMMCs. A, Neither SPE-7 (5 μg/mL, black bars) nor H1DNP-ε-26 IgE (5 μg/ml, gray bars) induced significant histamine release from BMMCs on stimulation with mouse HRF (n = 2). B, SPE-7 alone (5 μg/ml) induced phosphorylation of ERK1/2 in BMMCs (representative of 5 experiments). DNP-HSA, Dinitrophenylated human serum albumin. pERK, Phosphorylated ERK. Journal of Allergy and Clinical Immunology 2008 121, 1027-1033DOI: (10.1016/j.jaci.2007.12.1157) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Phosphorylation of ERK1/2 in basophils stimulated with buffer or various human IgEs (5 μg/mL) for 5 minutes (A) and 5 minutes and 15 minutes (B) is shown (n = 1). C, Histamine release to HrHRF and anti-IgE on cells sensitized with IgE+ (GG, black bars) and IgE− (F1491, gray bars; n = 3). D, ERK1/2 phosphorylation by serum or purified IgE. pERK, Phosphorylated ERK; STD, standard. Journal of Allergy and Clinical Immunology 2008 121, 1027-1033DOI: (10.1016/j.jaci.2007.12.1157) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Effects of human IgEs on IL-13 secretion in the absence of specific antigens. Basophil-enriched suspensions were stimulated with medium alone, human IgE+ or IgE− sera (5 μg/mL), purified IgE molecules (5 μg/mL), IL-3 (10 ng/mL), and A23187 (500 ng/mL) for 18 hours. Supernatants were harvested for IL-13 levels and measured by means of ELISA (n = 3 for all conditions, except n = 1 for pure IgE conditions). Journal of Allergy and Clinical Immunology 2008 121, 1027-1033DOI: (10.1016/j.jaci.2007.12.1157) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 A, Histamine release from basophils sensitized with IgE+ (7267, black bars) and IgE− (CB, gray bars; n = 1). B, ERK1/2 phosphorylation of human mast cells stimulated with buffer, 7267, or CB IgE (5 μg/mL) for 0, 5, and 15 minutes (representative of 5 experiments). C, ERK1/2 phosphorylation induced by human IgE in freshly purified and IL-3–primed human basophils (representative of 3). pERK, Phosphorylated ERK; STD, standard. Journal of Allergy and Clinical Immunology 2008 121, 1027-1033DOI: (10.1016/j.jaci.2007.12.1157) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 A, Human IgE alone augmented IL-3–induced IL-13 secretion in human basophils (n = 2). The synergistic effect of IgE on IL-3–induced IL-13 secretion only appeared when the dose of IgE reached 5 μg/mL. B, IgE (5 μg/mL) augmented the IL-3–induced IL-13 secretion approximately 2-fold compared with IL-3 alone (n = 3). ∗P < .05. Journal of Allergy and Clinical Immunology 2008 121, 1027-1033DOI: (10.1016/j.jaci.2007.12.1157) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions