HC70AL Oral Presentation Roman Groisberg Spring 2004

YES! Knockout in Salk line 006218 What is the structure of AT3G09735? Does a Knockout exist for AT3G09735? Knockout in Salk line 006218 Madison DNA pool 141 Madison Superpool 16

Where is AT3G09735 expressed in the Arabidopsis thaliana? Expressed at higher Levels in maturation stages! Where is the ortholog for AT3G09735 gene expressed in Scarlet Runner Bean? Expressed in all organs Except flowers.

What protein does AT3G09735 encode for? S1Fa DNA Binding protein What is the predicted structure of the protein? Coil-Helix-Coil-Helix-Coil Protein kinase C phosphorylation site N-myristoylation site What is the function of the protein? Gene repressor

What are the genotyping results? Salk plants 1-9: Fw/Rv primer +Tubulin T-DNA/RV +Tubulin Conclusion: No T-DNA insertions in plants 1-18

What are the phenotyping results? Wild type: Salk line 006218: Trichome Silique Flower Conclusion: No Difference

Cannot be determined without further research Is AT3G09735 important for seed development? Cannot be determined without further research What is next? Genotype more Salk Plants to find: homozygous T-DNA heterozygous T-DNA Genotype plants from other facilities that also have a knockout for at3g09735 Cross plants with knockouts in other S1Fa family genes and see if seeds develop with multiple S1Fa genes knocked out Crystalize protein to determine actual structure of S1Fa