ASSESSMENT OF MAJOR FACTORS AFFECTING TRIPLOID INDUCTION USING HYDROSTATIC PRESSURE IN THE EASTERN OYSTER (Crassostrea virginica) Name: Ian Sewell (MSc.

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Presentation transcript:

ASSESSMENT OF MAJOR FACTORS AFFECTING TRIPLOID INDUCTION USING HYDROSTATIC PRESSURE IN THE EASTERN OYSTER (Crassostrea virginica) Name: Ian Sewell (MSc candidate) Supervisor: Dr. Sarah Stewart - Clark Date: January 25, 2018

Research Problem/Project Goals MSX outbreak within the Bras D’or Lake occurred in 2002 [1] 95% decimation rate within the Bras D’or Lake [1] MSX still prevalent to this day To increase the resilience of the CB oyster industry through studying and implementing triploidy technology Fig 1: Haplosporidim nelsonii Fig 2: Spread of MSX within the Bras D’or Lake

Potential Solution MSX initial outbreak in the US dated back to the 1950s [2] The effects of MSX completely eradicated through polyploidy and breeding [2] Transfer of resistant/triploid/tetraploid oysters– Not a possibility Oysters in Canada are uniquely adapted to Canadian environmental and disease pressures Research Approach: Learn from them and implement these practises

Method & Results Deliverables Triploidy experiment 3x3 Multifactorial approach - 9 treatment combinations To determine the effect of: Pressure intensity (3 levels): 6000, 7000, 7500 PSI Pressure duration (3 levels): 3, 5, 10 mins 3. FIXED treatment initiation stage: 60% 1st Polar body [3] Larval ploidy level checked using flow cytometry (ABC hatcheries, VIMS) [4] Deliverables Triploid induction success in all treatments Up to 44% triploid induction rate No significant differences among treatments (P=0.229) Unintentional tetraploid success through late PB timing (70% 1st Polar body) Successful triploid groups deployed in the Bras D’or Lake for 2N/ 3N growth analysis

Acknowledgements & References I’d like to thank the AANS, DAL Aquaculture staff , VIMS – ABC Hatchery staff, my supervisor – Dr. Sarah Stewart-Clark, Scott Jeffrey, my academic committee, and all the involved farms for their assistance and participation throughout the project. References [1] Ford, S.E. and Haskin, H.H. 1982. History and Epizootiology of Haplosporidium nelsoni (MSX), an oyster pathogen in Delaware Bay, 1957 – 1980. J. Invertebr. Pathol. 40: 118 – 141 [2] Aquaculture Genetics and Breeding Technology Center. 2009. Virginia Institute of Marine Science, School of Marine Science, College of William and Mary. pp 28. [3] Allen Jr, S.K., Downing, S.L., Chew, K.K. 1989. Hatchery manual for producing triploid oysters. University of Washington Press, Seattle, WA, USA. [4] Allen Jr, S.K. 1983. Flow cytometry: assaying experimental polyploid fish and shellfish. Aquaculture. 33: 317 – 328

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