Molecular characterization of methicillin-resistant Staphylococcus epidermidis strains from bacteraemic patients S. Ibrahem, S. Salmenlinna, O. Lyytikäinen,

Slides:

Advertisements

Similar presentations

Molecular characterisation of Haemophilus influenzae type a and untypeable strains isolated simultaneously from cerebrospinal fluid and blood: novel use.

Advertisements

Gram-positive infections: lessons learnt and novel solutions

Detection of Panton–Valentine leukocidin gene in Staphylococcus aureus by LightCycler PCR: clinical and epidemiological aspects D. Johnsson, P. Mölling,

New epidemiology of Staphylococcus aureus infection in Asia

The use of Raman spectroscopy in the epidemiology of methicillin-resistant Staphylococcus aureus of human- and animal-related clonal lineages M.W.H.

Molecular characterization of methicillin-resistant Staphylococcus aureus isolated over a 2-year period in a Qatari hospital from multinational patients

S. M. Kotila, A. Virolainen, M. Snellman, S. Ibrahem, J. Jalava, O

Correlation between the activity of different fluoroquinolones and the presence of mechanisms of quinolone resistance in epidemiologically related and.

Detection of Panton–Valentine leukocidin gene in Staphylococcus aureus by LightCycler PCR: clinical and epidemiological aspects D. Johnsson, P. Mölling,

Clinical impact of antibiotic-resistant Gram-positive pathogens

Cost-effectiveness of universal MRSA screening on admission to surgery

Wide geographic distribution of a unique methicillin-resistant Staphylococcus aureus clone in Hungarian hospitals Herminia de Lencastre, Elena P. Severina,

Problems in diagnosis and treatment of Staphylococcus haemolyticus endocarditis in a hemodialysis patient Henk Louagie, Marc Struelens, Raf De Ryck,

Use of surveillance cultures and enteral vancomycin to control methicillin-resistant Staphylococcus aureus in a paediatric intensive care unit K. Thorburn,

Klebsiella pneumoniae: development of a mixed population of carbapenem and tigecycline resistance during antimicrobial therapy in a kidney transplant.

W. Witte, B. Pasemann, C. Cuny Clinical Microbiology and Infection

Community-acquired methicillin-resistant Staphylococcus aureus infections: an emerging threat in Spain A. Manzur, A.M. Dominguez, M. Pujol, M.P.M. González,

Molecular characterization and susceptibility of methicillin-resistant and methicillin- susceptible Staphylococcus aureus isolates from hospitals and the.

Isolation and characterisation of toxin A-negative, toxin B-positive Clostridium difficile in Dublin, Ireland D. Drudy, N. Harnedy, S. Fanning, R. O'Mahony,

Spa typing directly from a mecA, spa and pvl multiplex PCR assay—a cost-effective improvement for methicillin-resistant Staphylococcus aureus surveillance

Rapid detection, differentiation and typing of methicillin-resistant Staphylococcus aureus harbouring either mecA or the new mecA homologue mecALGA251

Replacement of methicillin-resistant Staphylococcus aureus clones in Hungary over time: a 10-year surveillance study T. Conceição, M. Aires-de-Sousa,

A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I–V K. Boye, M.D. Bartels, I.S. Andersen, J.A. Møller,

Clonal dissemination of epidemic methicillin-resistant Staphylococcus aureus in Belgium and neighboring countries A. Deplano, W. Witte, W.J. Van Leeuwen,

Dissemination of multidrug-resistant, class 1 integron-carrying Acinetobacter baumannii isolates in Taiwan L.-Y. Huang, T.-L. Chen, P.-L. Lu, C.-A. Tsai,

Low prevalence of methicillin-resistant Staphylococcus aureus with reduced susceptibility to glycopeptides in Belgian hospitals C. Nonhoff, O. Denis,

D. Esel, B. Sümerkan, S. Kocagöz Clinical Microbiology and Infection

A variant of the Southern German clone of methicillin-resistant Staphylococcus aureus is predominant in Croatia A. Budimir, R.H. Deurenberg, Z. Bošnjak,

Bacterial resistance—the clinical challenge

Lack of association between genotypes and haematogenous seeding infections in a large cohort of patients with methicillin-resistant Staphylococcus aureus.

PCR for the identification of methicillin-resistant Staphylococcus aureus (MRSA) strains using a single primer pair specific for SCCmec elements and the.

Panton–Valentine leukocidin-positive Staphylococcus aureus skin and soft tissue infections among children in an emergency department in Madrid, Spain

Gerda T. Noordhoek, Sitha A. Scheltinga, Paul Caesar, Leo M

Body site colonization in patients with community-associated methicillin-resistant Staphylococcus aureus and other types of S. aureus skin infections

Failure of bacteriophage typing to detect an inter-hospital outbreak of methicillin- resistant Staphylococcus aureus (MRSA) in Zagreb subsequently identified.

M. Aires-de-Sousa Clinical Microbiology and Infection

Characterisation of non-multiresistant methicillin-resistant Staphylococcus aureus (including EMRSA-15) in Kuwait Hospitals E.E. Udo, N. Al-Sweih, B.

Klebsiella pneumoniae: development of a mixed population of carbapenem and tigecycline resistance during antimicrobial therapy in a kidney transplant.

J. Price, N. Claire Gordon, D. Crook, M. Llewelyn, J. Paul

Y. Tveten, A. Jenkins, A.G. Allum, B.-E. Kristiansen

Prevalence and genetic diversity of Staphylococcus aureus small-colony variants in cystic fibrosis patients S. Yagci, G. Hascelik, D. Dogru, U. Ozcelik,

B.J.A. Rijnders, E. van Wijngaerden, J. van Eldere, W.E. Peetermans

A. Gelosia, L. Baldassarri, M. Deighton, T. van Nguyen

High-resolution melting analysis of the spa locus reveals significant diversity within sequence type 93 methicillin-resistant Staphylococcus aureus from.

Methicillin-resistant Staphylococcus aureus (MRSA) with reduced susceptibility to glycopeptides (GISA) in 63 French general hospitals G.L. Cartolano,

Identification of widespread, closely related Acinetobacter baumannii isolates in Portugal as a subgroup of European clone II G. Da Silva, L. Dijkshoorn,

P.M. Hawkey Clinical Microbiology and Infection

Increased incidence of methicillin-resistant Staphylococcus aureus ST80, novel ST125 and SCCmecIV in the south-eastern part of Norway during a 12-year.

Strain relatedness of meticillin-resistant Staphylococcus aureus isolates recovered from patients with repeated bacteraemia C-H. Liao, C-C. Lai, S-Y.

Predictive factors for mortality in patients with methicillin-resistant Staphylococcus aureus bloodstream infection: impact on outcome of host, microorganism.

Methicillin-susceptible Staphylococcus aureus clone related to the early pandemic phage type 80/81 causing an outbreak among residents of three occupational.

Molecular characterisation of a dominant methicillin-resistant Staphylococcus aureus (MRSA) clone in a Mexican hospital (1999–2003) G. Echániz-Aviles,

Community-associated Staphylococcus aureus infections and nasal carriage among children: molecular microbial data and clinical characteristics G. Sdougkos,

Epidemiological relatedness of methicillin-resistant Staphylococcus aureus from a tertiary hospital and a geriatric institution in Spain E. Ruiz de Gopegui,

G.M. Rossolini, E. Mantengoli Clinical Microbiology and Infection

Methicillin-resistant Staphylococcus saprophyticus in Sweden carries various types of staphylococcal cassette chromosome mec (SCCmec) B. Söderquist,

Rise and subsequent decline of community-associated methicillin resistant Staphylococcus aureus ST30-IVc in Copenhagen, Denmark through an effective search.

Evolutionary relationships between sporadic and epidemic strains of healthcare- associated methicillin-resistant Staphylococcus aureus M. Hallin, O. Denis,

Predominance of staphylococcal cassette chromosome mec (SCCmec) type IV among methicillin-resistant Staphylococcus aureus (MRSA) in a Swedish county and.

J. Wilson, S. Elgohari, D. M. Livermore, B. Cookson, A. Johnson, T

Usefulness of double locus sequence typing (DLST) for regional and international epidemiological surveillance of methicilin-resistant Staphylococcus aureus

A. Manzur, F. Gudiol Clinical Microbiology and Infection

Comparative genomic analysis of European and Middle Eastern community-associated methicillin-resistant Staphylococcus aureus (CC80:ST80-IV) isolates by.

B.A. Cunha Clinical Microbiology and Infection

G.C. Schito Clinical Microbiology and Infection

D.A. Robinson, M.C. Enright Clinical Microbiology and Infection

Microarray-based characterisation of a Panton–Valentine leukocidin-positive community- acquired strain of methicillin-resistant Staphylococcus aureus

Genetic diversity of community-associated methicillin-resistant Staphylococcus aureus in southern Stockholm, H. Fang, G. Hedin, G. Li, C.E.

Combination of pulsed-field gel electrophoresis (PFGE) and single-enzyme amplified fragment length polymorphism (SAFLP) for differentiation of multiresistant.

The origin of a methicillin-resistant Staphylococcus aureus isolate at a neonatal ward in Sweden—possible horizontal transfer of a staphylococcal cassette.

Presentation transcript:

Molecular characterization of methicillin-resistant Staphylococcus epidermidis strains from bacteraemic patients S. Ibrahem, S. Salmenlinna, O. Lyytikäinen, M. Vaara, J. Vuopio-Varkila Clinical Microbiology and Infection Volume 14, Issue 11, Pages 1020-1027 (November 2008) DOI: 10.1111/j.1469-0691.2008.02080.x Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 SCCmec—multiplex patterns of methicillin-resistant Staphylococcus epidermidis (MRSE). Methicillin-resistant Staphylococcus aureus (MRSA) strains (positive controls): SCCmec type IA (loci A, G and D), SCCmec type II (loci G, D, B and C), SCCmec type IIIA (loci F, E and C), SCCmec type IV (locus D). Internal control: mecA. MRSE strains: P1, IIIA (loci F, E and C) + IVA (loci G and D); P2, IIIB (locus C) + IV (locus D); P3, IIIB (;ocus C) + IVA (locus G without locus D); P4, IIIA (loci F, E and C) + IV (locus D); P5, IIIB (locus C) + IVA (loci G and D); P6, IIIA (loci F and C, without locus E) + IV (locus D); and IA. The sizes of amplicons are: (A) 495 bp, (B) 284 bp, (C) 209 bp, (D) 342 bp, (E) 243 bp, (F) 414 bp, (G) 381 bp and (mecA) 160 bp. M, molecular weight marker. Clinical Microbiology and Infection 2008 14, 1020-1027DOI: (10.1111/j.1469-0691.2008.02080.x) Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 (a) Schematic structures of methicillin-resistant Staphylococcus aureus (MRSA) SCCmec elements of type III and type IVA based on the findings of Chongtrakool et al. [19] and Oliveira and de Lencastre [15]. (b) Hypothetical structures and arrangements of methicillin-resistant Staphylococcus epidermidis (MRSE) SCCmec patterns (P1–P6) based on data provided in Tables 1–3. Primers highlighted in green yielded the amplified fragment between IS1272 and other elements, and the primers highlighted in red did not yield amplicons. Horizontal arrows within the cassette indicate the direction of SCCmec elements. Loci defined by multiplex PCR are indicated by arrows and appropriate letters (A–G). Unknown regions are indicated by broken lines. Clinical Microbiology and Infection 2008 14, 1020-1027DOI: (10.1111/j.1469-0691.2008.02080.x) Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 3 Pulsed-field gel electrophoresis (PFGE) dendrogram of the Finnish methicillin-resistant Staphylococcus epidermidis strains. The scale bar at the top of the dendrogram indicates similarity. Clinical Microbiology and Infection 2008 14, 1020-1027DOI: (10.1111/j.1469-0691.2008.02080.x) Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions