You Can Count on This: Barcoded Hematopoietic Stem Cells

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Presentation transcript:

You Can Count on This: Barcoded Hematopoietic Stem Cells Hanno Glimm, Claudia R. Ball, Christof von Kalle Cell Stem Cell Volume 9, Issue 5, Pages 390-392 (November 2011) DOI: 10.1016/j.stem.2011.10.013 Copyright © 2011 Elsevier Inc. Terms and Conditions

Figure 1 Clonal Tracking of Virally Marked Stem Cell Clones The progeny of individual stem cell clones that were marked by integrating viral vectors can be tracked after transplantation using two strategies. Barcodes introduced into the marking vector can be efficiently amplified genome-wide. This strategy allows an unbiased quantification of clonal contribution (left). On the other hand, the exact genomic location of vector integration enables deciphering the influence of vector integration on the stem cell clones' biology (right). LTR, long terminal repeat; R, restriction enzyme motif; red arrows, PCR primers; colored lines, PCR amplicons. Cell Stem Cell 2011 9, 390-392DOI: (10.1016/j.stem.2011.10.013) Copyright © 2011 Elsevier Inc. Terms and Conditions