Hyperglycemia limits experimental aortic aneurysm progression

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Presentation transcript:

Hyperglycemia limits experimental aortic aneurysm progression Noriyuki Miyama, MD, PhD, Monica M. Dua, MD, Janice J. Yeung, MD, Geoffrey M. Schultz, MD, Tomoko Asagami, MD, PhD, Eiketsu Sho, MD, PhD, Mien Sho, MD, PhD, Ronald L. Dalman, MD Journal of Vascular Surgery Volume 52, Issue 4, Pages 975-983 (October 2010) DOI: 10.1016/j.jvs.2010.05.086 Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 1 Aortic diameters in (A) PPE vs PPE-DM mice (†P = .0016, POD 7; ‡P < .0001, POD 14) and (B) ANG vs ANG-DM mice (*P = .0474, POD 14; *P = .0472, POD 21). The range bars show the standard deviation. ANG, angiotensin II; DM, diabetes mellitus; POD, postoperative day; PPE, porcine pancreatic elastase. Journal of Vascular Surgery 2010 52, 975-983DOI: (10.1016/j.jvs.2010.05.086) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 2 Representative images of CD31 staining in (A) PPE and (B) PPE-DM (original magnification ×400). AD, adventitia; Ao, aorta; M, media. C, Number of medial and adventitial CD31+ vessels (A and B, black arrows) per high power field (HPF). The range bars show the standard deviation. *P < .0229 vs PPE; small circles represent individual data points, large circle represents mean of cohort. D, Correlation between neovessel density and aortic diameter, Y = 29.68X – 17.86; correlation coefficient, R = 0.794; P < .0001; PPE (n = 9), PPE-DM (n = 10). DM, Diabetes mellitus; PPE, porcine pancreatic elastase. Journal of Vascular Surgery 2010 52, 975-983DOI: (10.1016/j.jvs.2010.05.086) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 3 Representative images of MAC-2 staining in (A) PPE and (B) PPE-DM (original magnification ×200). M, Media. C, Number of mural MAC-2+ cells per cross-section are shown. The range bars show the standard deviation. *P = .0325 vs PPE. D, Correlation between macrophage density and aortic diameter, Y = 418.04X – 284.35; correlation coefficient, R = 0 .777, P < .0001. E, Correlation between macrophage and neovessel density, Y = 9.53X + 41.51; correlation coefficient R = .662; P < .01. Data for D and E are from PPE (n = 9) and PPE-DM (n = 10) mice. DM, Diabetes mellitus; PPE, porcine pancreatic elastase. Journal of Vascular Surgery 2010 52, 975-983DOI: (10.1016/j.jvs.2010.05.086) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 4 A representative image of (A) zymographic and densitometric analysis of (B) pro-MMP-9, (C) pro-MMP-2, and (D) active MMP-2. *P = .0259 vs PPE. Representative images of Elastica-Masson-stained aortic cross-sections in (E) PPE (F) and PPE-DM (original magnification ×200), and (G) the ratio of stained-elastin-area to media-area. *P = .0209 vs PPE. DM, Diabetes mellitus; MMP, matrix metalloproteinase; PPE, porcine pancreatic elastase. The range bars show the standard deviation. Journal of Vascular Surgery 2010 52, 975-983DOI: (10.1016/j.jvs.2010.05.086) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 5 Comparison of aortic diameter at baseline and after porcine pancreatic elastase (PPE) infusion in PPE (n = 6; white bar), PPE-DM (n = 6; grey bar) and PPE-DM + insulin (n = 6; black bar) mice (POD 7 *P =.0014, #P <.0001; POD 14 *P =.0036, #P = .0002). The range bars show the standard deviation. DM, Diabetes mellitus; POD, postoperative day. Journal of Vascular Surgery 2010 52, 975-983DOI: (10.1016/j.jvs.2010.05.086) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 6 Comparison of peritoneal macrophages in lavage solution from diabetes mellitus (DM) mice and control mice with thioglycollate-induced peritonitis. *P = .0171, #P < .0001. The range bars show the standard deviation. NS, No significance; thio, thioglycollate; VEGF, vascular endothelial growth factor; WT, wild type. Journal of Vascular Surgery 2010 52, 975-983DOI: (10.1016/j.jvs.2010.05.086) Copyright © 2010 Society for Vascular Surgery Terms and Conditions