The Microscope

The Microscope Enables you to see things that are too small to be seen with the unaided eye. Allows you to look more closely at the finer details of things.

What the microscope sees What you see What the microscope sees Blood

What the microscope sees What you see What the microscope sees Fly

What the microscope sees What you see What the microscope sees Leaf

What the microscope sees What you see What the microscope sees Eyelash

What the microscope sees What you see What the microscope sees Strawberry

Microscope Parts Eyepiece (10X) Coarse Adjustment Body Tube Fine Adjustment Nosepiece Arm Scanning Objective (4X) High Power Objective (40X) Stage Stage Clips Base Diaphragm Light Source

Parts at Work Eyepiece – What you look through. Objectives by itself enlarges the specimen by 10X Objectives Scanning Power (4X) = to locate and focus the specimen, allows you to see the largest amount of a specimen. (Red) Low Power (10X) = to examine details (Yellow) High Power (40X) = to examine the finest details (Blue)

Parts at Work Coarse Adjustment – to focus in the specimen under scanning and low power. Fine Adjustment – to focus in the specimen under high power ONLY. Arm & Base – used to carry the microscope. Nosepiece – holds the objective lenses.

Parts at Work Stage & Stage Clips – holds the slide with specimen securely. Diaphragm – adjusts the amount of light shining through specimen. Light Source – provides light that shines through the specimen.

Magnifications Part Magnification Eyepiece Scanning Objective Low Power Objective High Power Objective 10X 4X 10X 40X

Total Magnification The total amount a specimen is magnified due to the combined magnifications of the eyepiece and objective lenses being used. Eyepiece Objective Total Magnification X = 10X 5X 50X 15X 15X 225X 10X 10X 100X 20X 40X 800X

Total Magnifications Which total magnification would allow you to see: Eyepiece Objective Total Magnification 10 4 40 100 400 Which total magnification would allow you to see: The largest amount of the total specimen? The finest details of the specimen? Which set of lenses would you use to find and focus a specimen? 40X 400X 40X

Total Magnification B. A. C. _____ 1. Scanning Power (40X) Used to find a specimen _____ 2. Low Power (100X) To view more detail of the specimen. _____ 3. High Power (400X) To view the greatest detail of the specimen. A. B. C. C.

Field of View The diameter of the circle of light that you see when looking through the eyepiece is called the Field of View. What is the field of view as seen in the image? _______ cm or _______ mm 1.5 15

Field of View As the power of the microscope becomes greater the field of view gets smaller. When measuring field of view always read it from the diameter. Scanning Power: (40X) High Power: (400X) 4.0 mm .5 mm

Field of View If every = 1mm, what would be the diameter of this field of view? If every = 1mm, what would be the approximate size of letter “e”? A. 6 mm B. 2 mm