Cell Structure The Microscope

Introduction - Microscope Discovery of Micro-organisms Allows us to look at cells Has lead to discoveries in many diseases Cytology - the study of cells Light Microscope Electron Microscope Beams of Light Beams of electrons X 1500 X 500,000

The Light Microscope You Must be able to label a microscope & state its functions

Functions of Microscope Eyepiece lens - magnifies the image Course adjustment - rough adjustment Fine adjustment - precise focusing Nosepiece - revolves lens into position Objective lens - magnifies image Stage - holds the slide Light source - sends light through the slide Clips - hold the slide in place

Basic Cell Structure Plant or Animal Cells???

Cell Ultra – Structure (Animal)

Cell Ultra – Structure (Plant)

Cell Membrane Phospholipids and and proteins bi-layer FUNCTIONS: Controls movement of substances in & out of cell. Production of anti-bodies Support Structure

Cell Wall Only found in plant cells. Made by the cytoplasm. Made of cellulose Fully Permeable Functions Support Protection Middle Lamella: A chemical material which 'glues' plant cells together. It is produced during cell division.

The Nucleus The nucleus is the control centre of the cell. Structure Nuclear membrane Nuclear pores Nucleoli - manufacture proteins Chromosomes/chromatin

The Nucleus The nucleus is the control centre of the cell. Functions Cell division - mitosis/meiosis Protein synthesis Controls activities of the cell

Mitochondria Most are found in active cells like muscle cells, nerve cells etc… Used in plant and animal cells for respiration. Produces a large number of enzymes. matrix cristae

Chloroplasts Only found in plant cells Contain pigment chlorophyll Used during photosynthesis to absorb sunlight

Ribosomes Produced by the nucleolus in the nucleus. Tiny, but very numerous. Either floating freely in cytoplasm or attached to endoplasmic reticulum. Protein synthesis. Sometimes they may be attached together in a group called a 'polysome'.

Centrioles Mainly found in animal cells. Occur in pairs near the nucleus. Function Cell division

Vacuole Mainly in plant cells. Found in some animal cells such as Amoeba. Controls the water content of the cell. Contains food/excretory products (sap)

Endoplasmic Reticulum A network of canals in the cells which serves as a transport system. Store food materials such as proteins, fats, carbohydrates, vitamins and minerals. Sometimes there are ribosomes attached to the outside of the E.R. - these are called Rough Endoplasmic Reticulum. When there are no ribosomes attached they are called Smooth Endoplasmic Reticulum.

Golgi Apparatus: Sometimes called Golgi Bodies. Functions are bile production, mucous and synovial fluid secretion, eye pigment, enamel, keratin (nails and hair protein) and the production of cell membranes

Differences in Plant & Animal Cells Plants Animals Cell Walls No Cell Walls Chloroplasts No chloroplasts Chlorophyll No chlorophyll Large Vacuoles Small (if any) vacuoles

Prokaryotic and Eukaryotic Cells cells that contain a nucleus bound by a nuclear membrane. They also contain many membrane bound structures called organelles.

Prokaryotic and Eukaryotic Cells Prokaryotic cells Cells that do not have a nucleus bound by a nuclear membrane. Instead of having DNA fixed on chromosomes their genetic information is on a circular loop called a plasmid. Example - bacteria cell.

Mandatory Experiments To prepare an examine a plant cell, stained & unstained using a light microscope a. Plant cell – onion, get the thin epidermis between layers of the onion to allow light pass through b. Stain – iodine, shows up membranes c. Apply cover slip at an angle to prevent air bubbles d. Start the procedure at low power to find the cell and prevent damage to the microscope e. Be able to draw the results

Mandatory Experiments To prepare and examine an animal cell using a light microscope a. Animal cell – cheek cell b. Spread the cells out thinly to allow the light to pass though c. Dye – methylene blue, stains the nucleus dark blue d. Apply cover-slip at an angle to prevent air bubbles e. Start with low objective lens f. Use fine focus on the high power to prevent damage to the microscope g. Be able to draw results