Raman Spectroscopy Detection of Phytic Acid in Plant Seeds Reveals the Absence of Inorganic Polyphosphate  Bernadett Kolozsvari, Steven Firth, Adolfo Saiardi  Molecular Plant  Volume 8, Issue 5, Pages 826-828 (May 2015) DOI: 10.1016/j.molp.2015.01.015 Copyright © 2015 The Author Terms and Conditions

Figure 1 Comparison of Raman Spectra of polyP and Phytic Acid as Solids or in Solution and Analysis of Different Layers of Wheat Seeds. (A) The Raman spectrum for polyP shows an intense phosphate stretching band at 1154 cm−1. (B) The Raman spectrum of a 100 mM solution of polyP shows significant intensity of the stretching phosphate vibrations at 1152 cm−1. (C) The Raman spectrum of phytic acid, demonstrating various phosphate stretching peaks between 700 and 1400 cm−1. The central phosphate band was detected at 1007 cm−1. (D) The Raman spectrum from a 10 mM phytic acid solution. The phosphate band is seen at 1010 cm−1 and the spectrum shows similar features as for solid phytic acid. (E) Confocal microscopy image (Leica SP5) was taken with a 63×/0.75 oil objective and shows the three different layers of wheat seeds (pericarp, aleurone cells and endosperm). (F) The Raman spectra recorded from untreated (in red) and phytase-treated (in black) pericarp. No Raman signal of phytic acid was present in these cells. (G and H) Raman spectra recorded from untreated (in red) and treated with phytase (in black) aleurone cells (G) and endosperm (H) revealed the Raman signal of phytic acid. No phosphate bands were observed after the 1-h phytase treatment. The spectra are representative of five scans from three independent cells for each layer. Scale bar, 10 μm. Molecular Plant 2015 8, 826-828DOI: (10.1016/j.molp.2015.01.015) Copyright © 2015 The Author Terms and Conditions