by Rafael Fonseca, Richard J. Bailey, Gregory J. Ahmann, S

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Genomic abnormalities in monoclonal gammopathy of undetermined significance by Rafael Fonseca, Richard J. Bailey, Gregory J. Ahmann, S. Vincent Rajkumar, James D. Hoyer, John A. Lust, Robert A. Kyle, Morie A. Gertz, Philip R. Greipp, and Gordon W. Dewald Blood Volume 100(4):1417-1424 August 15, 2002 ©2002 by American Society of Hematology

Pictures with both the normal and abnormal patterns for the break-apart and fusion strategies.In all cases the clonal PCs are identified by the intense blue fluorescence of the cytoplasm as detected by the cIg-FISH procedure. Pictures with both the normal and abnormal patterns for the break-apart and fusion strategies.In all cases the clonal PCs are identified by the intense blue fluorescence of the cytoplasm as detected by the cIg-FISH procedure. (A) A PC with the normal configuration for the VH (red signal) and CH probes (green signal). Arrows point to the closely associated pairs of signals indicative of a normal pattern. (B) Segregation (break-apart) of the VH (green) and CH (red) probes consistent with a 14q32 translocation. (C) A cell with fusion signals (arrows) resulting from comigration of the pools of probes at 14q32 (green) and 4p16.3 (red) consistent with a t(4;14)(p16.3;q32). (D) The fusion signal (arrows) resulting from comigration of the pools of probes at 14q32 (green) and 16q23 (red) consistent with a t(14;16)(q32;q23). (× 100 magnification, Leica DMRXA microscope [Wetzlar, Germany]). Rafael Fonseca et al. Blood 2002;100:1417-1424 ©2002 by American Society of Hematology

Genetic up-regulation of FGFR3 and cyclin D1 Genetic up-regulation of FGFR3 and cyclin D1.The left upper panel shows a PC with the intense cytoplasmic fluorescence for the FGFR3 (FITC) and is counterstained with DAPI. This patient had a t(4;14)(p16.3;q32) detected by cIg-FISH. Genetic up-regulation of FGFR3 and cyclin D1.The left upper panel shows a PC with the intense cytoplasmic fluorescence for the FGFR3 (FITC) and is counterstained with DAPI. This patient had a t(4;14)(p16.3;q32) detected by cIg-FISH. The PC in the upper right panel shows no reactivity to the FGFR3antibody (no t(4;14)(p16.3;q32) detected by cIg-FISH). The lower left panel depicts a PC with intense nuclear positivity for cyclin D1 by immunohistochemistry (light H-E counterstain). This patient also had the t(11;14)(q13;q32) detected. Inset shows nuclear localization in enlarged cell. The lower right panel shows a PC of a patient negative for cyclin D1 and for the t(11;14)(q13;q32) by FISH (100 × magnification, Leica DMRXA microscope). Rafael Fonseca et al. Blood 2002;100:1417-1424 ©2002 by American Society of Hematology