Proinflammatory gene expression in patients undergoing mitral valve surgery and maze ablation for atrial fibrillation Feng-Chun Tsai, MD, Gwo-Jyh Chang,

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Presentation transcript:

Proinflammatory gene expression in patients undergoing mitral valve surgery and maze ablation for atrial fibrillation Feng-Chun Tsai, MD, Gwo-Jyh Chang, PhD, Yu-Juei Hsu, MD, PhD, Yuan-Min Lin, PhD, Yun-Shien Lee, PhD, Wei-Jan Chen, MD, PhD, Chi-Tai Kuo, MD, Yung-Hsin Yeh, MD The Journal of Thoracic and Cardiovascular Surgery Volume 151, Issue 6, Pages 1673-1682.e5 (June 2016) DOI: 10.1016/j.jtcvs.2015.12.003 Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Significant differentially expressed proinflammatory genes in left atrial appendages (A) between sinus rhythm (SR) and atrial fibrillation (AF) and (B) between recurrent AF after the maze procedure (AF-AF) and maintained SR after the maze procedure (AF-SR). The results are presented as log 2 (SR/AF) and log 2 (AF-SR/AF-AF), respectively. LA, Left atria. The Journal of Thoracic and Cardiovascular Surgery 2016 151, 1673-1682.e5DOI: (10.1016/j.jtcvs.2015.12.003) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Comparison between quantitative polymerase chain reaction (qPCR) and nCounter Human Inflammation Array (NanoString Technologies, Seattle, Wash) (A) between atrial fibrillation (AF) and sinus rhythm (SR) and (B) between recurrent AF after the maze procedure (AF-AF) and maintained SR after the maze procedure (AF-SR). Data are expressed as log 2 fold change. Genes were selected by virtue of significant differences between groups in inflammation array analysis. The results were presented as log 2 (SR/AF) and log 2 (AF-SR/AF-AF), respectively. For qPCR, data are presented as box-and-whisker plot. N = 5 to 7 samples for each gene. LA, Left atria. The Journal of Thoracic and Cardiovascular Surgery 2016 151, 1673-1682.e5DOI: (10.1016/j.jtcvs.2015.12.003) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 A, Western blot analysis of SHC1, RHOA, PDGFA, and TRAF2 between sinus rhythm (SR) and atrial fibrillation (AF). B, Western blot analysis of CCL4, IL15, phospho- and total STAT1, RAC1, and NFATc3 between recurrent AF after the maze procedure (AF-AF) and maintained SR after the maze procedure (AF-SR). The relative expression levels of proteins corresponding to GAPDH were quantified by densitometry and normalized to A, SR and B, AF-SR, respectively, which was set at 1.0. N = 3 to 5 for each group. *P < .05. The Journal of Thoracic and Cardiovascular Surgery 2016 151, 1673-1682.e5DOI: (10.1016/j.jtcvs.2015.12.003) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 Confocal immunohistochemistry analysis of reactive oxidative stress (ROS), transforming growth factor beta 1 (TGF-β1), collagen, and nuclear factor of activated T-cells cytoplasmic 3 (NFATc3) between sinus rhythm (SR), maintained sinus rhythm after maze procedure (AF-SR) and recurrent atrial fibrillation after maze procedure (AF-AF). The relative intensity is normalized to SR, which is set at 1.0. N = 3 to 5 samples for each group. *Versus SR. #Versus AF-SR (P < .05). The Journal of Thoracic and Cardiovascular Surgery 2016 151, 1673-1682.e5DOI: (10.1016/j.jtcvs.2015.12.003) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 5 Activation of HL-1 atrial myocyte regulated the expression of SHC1, RHOA, PDGF-A, and TRAF2 at protein level at a frequency-dependent manner. The relative expression levels of proteins corresponding to GAPDH were quantified by densitometry and normalized to 1-Hz pacing, which was set at 1.0. N = 4 for each group. *P < .05 versus 1 Hz. The Journal of Thoracic and Cardiovascular Surgery 2016 151, 1673-1682.e5DOI: (10.1016/j.jtcvs.2015.12.003) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 6 Regression analysis between left atrial diameter (LAD) and (A) RHOA and (B) STAT1. SR, Sinus rhythm; AF-SR, maintained sinus rhythm after maze procedure; AF-AF, AF recurrence after maze procedure. The Journal of Thoracic and Cardiovascular Surgery 2016 151, 1673-1682.e5DOI: (10.1016/j.jtcvs.2015.12.003) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure 7 Receiver operator curve analysis of PTK2 in recurrent atrial fibrillation after the maze procedure and maintained sinus rhythm after the maze procedure. AUC, Area under the curve. The Journal of Thoracic and Cardiovascular Surgery 2016 151, 1673-1682.e5DOI: (10.1016/j.jtcvs.2015.12.003) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure E1 A, Representative examples of Western blot of SHC1, RHOA, PDGFA, and TRAF2 between sinus rhythm (SR) and atrial fibrillation (AF) and B, Representative examples of Wetern blot of CCL4, IL15, phospho-, and total STAT1, RAC1, and nuclear factor of activated T-cells cytoplasmic 3 (NFATc3) between recurrent atrial fibrillation after the maze procedure (AF-AF) and maintained sinus rhythm after maze procedure (AF-SR). The Journal of Thoracic and Cardiovascular Surgery 2016 151, 1673-1682.e5DOI: (10.1016/j.jtcvs.2015.12.003) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure E2 Representative examples of confocal immunohistochemistry of reactive oxidative stress (ROS), transforming growth factor beta 1 (TGF-β1), collagen, and nuclear factor of activated T-cells cytoplasmic 3 (NFATc3) between sinus rhythm (SR), maintained sinus rhythm after maze procedure (AF-SR) and recurrent atrial fibrillation after maze procedure (AF-AF). The Journal of Thoracic and Cardiovascular Surgery 2016 151, 1673-1682.e5DOI: (10.1016/j.jtcvs.2015.12.003) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Figure E3 Representative examples of Western blot of SHC1, RHOA, PDGF-A, and TRAF2 in HL-1 atrial myocyte after pacing. The Journal of Thoracic and Cardiovascular Surgery 2016 151, 1673-1682.e5DOI: (10.1016/j.jtcvs.2015.12.003) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions

Gene expression in atrial fibrillation and its recurrence after surgical ablation. The Journal of Thoracic and Cardiovascular Surgery 2016 151, 1673-1682.e5DOI: (10.1016/j.jtcvs.2015.12.003) Copyright © 2016 The American Association for Thoracic Surgery Terms and Conditions