Volume 42, Issue 4, Pages (October 2002)

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Date of download: 6/1/2016 Copyright © 2016 American Medical Association. All rights reserved. From: Expression of Matrix Metalloproteinases and Their.

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Volume 42, Issue 4, Pages 398-406 (October 2002) Different mRNA and Protein Expression of Matrix Metalloproteinases 2 and 9 and Tissue Inhibitor of Metalloproteinases 1 in Benign and Malignant Prostate Tissue Ralf Lichtinghagen, Petra B Musholt, Michael Lein, Andreas Römer, Birgit Rudolph, Glen Kristiansen, Steffen Hauptmann, Dietmar Schnorr, Stefan A Loening, Klaus Jung European Urology Volume 42, Issue 4, Pages 398-406 (October 2002) DOI: 10.1016/S0302-2838(02)00324-X

Fig. 1 Gelatin zymography of standards of MMP-2 and MMP-9 and of protein extracts of noncancerous and cancerous tissue samples of the same prostate obtained at radical prostatectomy. Lanes 1–4 contained standards of MMP-2 (72kD) and of MMP-9 (92kD): 12, 9.6, 6.0, 4.8ng of purified proMMP-2 and 4.8, 4.0, 2.4, 1.7ng of purified proMMP-9, respectively, (further details see Section 2) were loaded to make calibration curves. Lanes 5–7: typical digestion patterns of the extracts from matched pairs of noncancerous (“a” behind the number) and cancerous (“b” behind the number) parts of three prostates obtained at radical prostatectomy. Equal protein amounts (12μg) were loaded. MMP-9 were generally higher in cancerous than in noncancerous samples. European Urology 2002 42, 398-406DOI: (10.1016/S0302-2838(02)00324-X)

Fig. 2 Scatter plots of (A) MMP-2, (B) MMP-9, (C) TIMP-1, and the ratios of (D) MMP-2/TIMP-1 and (E) MMP-9/TIMP-1 measured as mRNA expression by quantitative RT-PCR in (PN) noncancerous and (PC) cancerous tissue samples of the same prostate obtained after radical prostatectomy. Horizontal lines indicate mean values. Statistical differences between the malignant and benign tissue samples are indicated as p-values for paired data. European Urology 2002 42, 398-406DOI: (10.1016/S0302-2838(02)00324-X)

Fig. 3 Scatter plots of (A) MMP-2, (B) MMP-9, (C) TIMP-1, and the ratios of (D) MMP-2/TIMP-1 and (E) MMP-9/TIMP-1 measured as protein expression by zymography and ELISA in (PN) noncancerous and (PC) cancerous tissue samples of the same prostate obtained after radical prostatectomy. The concentrations analyzed were related to the total tissue protein. Horizontal lines indicate mean values. Statistical differences between the malignant and benign tissue samples are indicated as p-values for paired data. European Urology 2002 42, 398-406DOI: (10.1016/S0302-2838(02)00324-X)

Fig. 4 Correlation matrix between the mRNA expression and protein expression for (A) MMP-2, (B) MMP-9, and (C) TIMP-1 in human prostatic tissue. Spearman rank correlation coefficients were calculated from 34 tissue samples. The corresponding significance levels are indicated for each correlation. European Urology 2002 42, 398-406DOI: (10.1016/S0302-2838(02)00324-X)