Rafael Marques da Silva, DDS, Dominique A. Caugant, PhD, Emenike R. K

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Bacterial diversity in aortic aneurysms determined by 16S ribosomal RNA gene analysis Rafael Marques da Silva, DDS, Dominique A. Caugant, PhD, Emenike R.K. Eribe, PhD, Jørn A. Aas, DDS, PhD, Per S. Lingaas, MD, Odd Geiran, MD, PhD, Leif Tronstad, DMD, PhD, Ingar Olsen, DDS, PhD Journal of Vascular Surgery Volume 44, Issue 5, Pages 1055-1060 (November 2006) DOI: 10.1016/j.jvs.2006.07.021 Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Fig 1 Polymerase chain reaction products from aortic aneurysm samples using eubacterial primers to amplify approximately 500 bp of the 16S rRNA gene. Lane M, Molecular weight marker (1Kb DNA Ladder, Invitrogen). Lanes 1 to 10, DNA extracted from patients’ aneurysm samples. Lanes N, negative controls (sterile water). Lane P, positive control (purified DNA from E. coli). Journal of Vascular Surgery 2006 44, 1055-1060DOI: (10.1016/j.jvs.2006.07.021) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions

Fig 2 Phylogenetic tree of representative 16S rDNA sequences obtained from 10 aortic aneurysm samples. The sequences were aligned using ClustalV, and the unrooted tree was constructed using the DNASTAR program, showing two major clusters at 10% nucleotide sequence difference. Journal of Vascular Surgery 2006 44, 1055-1060DOI: (10.1016/j.jvs.2006.07.021) Copyright © 2006 The Society for Vascular Surgery Terms and Conditions