Single‐molecule tracking in living cells.

Slides:



Advertisements
Similar presentations
In what phase is cell #1?. Name this part of the microscope.
Advertisements

Cytoplasmic Regulation of the Movement of E-Cadherin on the Free Cell Surface as Studied by Optical Tweezers and Single Particle Tracking: Corralling and.
Levels of organization
Tuning the response of the DPI
Culture type shapes drug‐induced growth arrest in ovarian cancer patient cells Culture type shapes drug‐induced growth arrest in ovarian cancer patient.
Abundance of proteins matching selected subcellular locations and functions in CaCo‐2 cells. Abundance of proteins matching selected subcellular locations.
Mutant EGFR complexes in lung cancer cell lines and immortalized human bronchial epithelial cells. Mutant EGFR complexes in lung cancer cell lines and.
Statistical analysis of the influence of network regulators on topology clusters in the oleate network. Statistical analysis of the influence of network.
A kinetic model reconciles observed ERK phosphorylation, localization, and activity responses A Schematic of a simple kinetic model including cytosolic.
(A) gToW growth phenotypes occur in the absence of leucine, and red squares indicate a significant growth‐rate defect as compared with the empty plasmid.
(A) Graph depicting interaction between the five regulatory motifs measured by synergy and cooccurrence. (A) Graph depicting interaction between the five.
Analysis of in silico flux changes along the exponential growth phase: (A) In silico flux changes from 24 to 36 h, from 36 to 48 h, and from 48 to 60 h.
Epistatic interactions and gene expression variation.
Heatmaps of the gene mutation distributions in oncogene‐signaling blocks for six representative cancer types. Heatmaps of the gene mutation distributions.
(C–F) Complete interaction networks (representing both baits and preys) for selected groups of baits. (C–F) Complete interaction networks (representing.
The TBON model. The TBON model. (A) Representative confocal images of E14Tg2A cells stained for Tcf3 (green), Nanog (red), Oct4 (magenta), and total β‐catenin.
Clustering of CD34+ single cells
Comparative analysis of RNA and protein profiles.
Highly correlating interaction profiles can predict functional similarity Highly correlating interaction profiles can predict functional similarity AROC.
Confirmation of pool data with isogenic culture
Dose response of pAGA1 and pFIG1 induction
Large‐scale image‐based CRISPR‐Cas9 gene perturbation profiling
Isolation of transcription elongation complexes from the 5′ and 3′ regions of a single gene Isolation of transcription elongation complexes from the 5′
Correlating protein to mRNA and proteins per mRNA ratios
Coupling immunophenotypes to Drop‐seq data
Network derived from large‐scale fractionation predicts 48 protein complexes and communities Network derived from large‐scale fractionation predicts 48.
Characterization of promoters relative to pAGA1
Dynamics and variability of SMAD2 signaling in single cells
Heat maps showing global relative growth phenotype and comparison between measured and predicted values. Heat maps showing global relative growth phenotype.
Networks of ERK substrates.
Alignment time for Clustal Omega (red), MAFFT (blue), MUSCLE (green) and Kalign (purple) against the number of sequences of HomFam test sets. Alignment.
Mutants of the Group III have differentially impaired induction of pAGA1 and pFIG1 Mutants of the Group III have differentially impaired induction of pAGA1.
Physical EGFR interactome generation and core network proteins identification. Physical EGFR interactome generation and core network proteins identification.
Feature‐based COG enrichment analysis
Changes to the growth conditions break the circuit by changing host gene expression Changes to the growth conditions break the circuit by changing host.
Dynamic changes of protein phosphorylation identify ERK1/2 substrates from cytosolic and nuclear compartments. Dynamic changes of protein phosphorylation.
Lysozyme expression in isolated Paneth cells and linage tracing of Notch1+ cells Lysozyme expression in isolated Paneth cells and linage tracing of Notch1+
Effect of the loss of Kar4 on the induction of various promoters
Patient organoids respond more diverse to drugs and with lower therapeutic potential than 2D cultured patient cells Patient organoids respond more diverse.
Dynamics and expression level of mating‐dependent promoters
Optimality principles in adaptive evolution.
DeathPro dyes Hoechst and PI do not affect organoid growth and drug responses DeathPro dyes Hoechst and PI do not affect organoid growth and drug responses.
Schematic representation of LuTHy
Allele‐specific expression at single‐cell resolution
Oxidative damage cell death pathway model.
Two‐way unsupervised uncentered unnormalized hierarchical clustering using a Pearson's correlation of the phenotypic profiles of 4281 nonessential genes.
Transcriptomic and epigenetic changes associated with Factor 1 in the scMT data Transcriptomic and epigenetic changes associated with Factor 1 in the scMT.
Categorizing cell type‐specific auxin responses.
Comparison of nuclear surface area of HeLa and RKO cells
Live‐cell imaging uncovers a slowly cycling drug‐resistant state involved in adaptation to RAF inhibition Live‐cell imaging uncovers a slowly cycling drug‐resistant.
Impact of growth phase on the Escherichia coli meltome and proteome
Simulation showing that the cell length variability of the entire population can mask abnormal cell length variability at a specific cell cycle period.
An integrated NHR network.
Subnetworks enriched for the hallmarks of cancer.
Stage‐dependent diurnal transcript changes.
Example transcript and protein patterns.
Distinct collagen structures in the upper and lower neonatal dermis (related to Fig 1)‏ Distinct collagen structures in the upper and lower neonatal dermis.
Reproducibility of DeathPro drug screens
The switch to galactose imposes a heavy cost after long‐term glucose repression (LTGR)‏ The switch to galactose imposes a heavy cost after long‐term glucose.
Melting behavior of protein complexes
Dynamic transcriptome analysis in yeast.
Experimental validation of predicted endocytosis functions in human.
SP3 provides an efficient means for preparing protein and peptide samples for MS analysis Schematic of the SP3 workflow in a single tube. SP3 provides.
The transcriptional behavior of GREB1 changes with estrogen dose and exhibits considerable cell‐to‐cell variation (see also Fig EV2 and Movie EV2)‏ The.
Perturbation of ligand depletion affects the ultrasensitivity of long‐term P‐Smad2 responses to different doses of TGF‐β stimulation. Perturbation of ligand.
Shape‐sensitivity profiles inferred using shape projection
Shapes.
Fraction of flux entering the PEP‐glyoxylate cycle as a function of hexose uptake rate in batch (A) and chemostat (B) cultures. Fraction of flux entering.
Changes in fibroblast activation and proliferation outside the wound and CHP staining in the wound bed (related to Fig 5)‏ Changes in fibroblast activation.
BRI1 signaling at the dividing cells restores overall root growth
Presentation transcript:

Single‐molecule tracking in living cells. Single‐molecule tracking in living cells. (A) Cy3–NGF (nerve growth factor) bound to the surface of a PC12 cell. The white line delineates the shape of the cell. (B) Single‐molecule trajectories of the receptor–ligand (Cy3–NGF) complex on the cell surface. The movement of the complex displayed repeated alternation between mobile and immobile phases. During immobile phases (yellow circles), the complex formed clusters with other complexes (red trajectories). Yasushi Sako Mol Syst Biol 2006;2:56 © as stated in the article, figure or figure legend