A Novel <b><i>ABO</i></b> Gene Variant Leads to Discrepant Results in Forward/Reverse and Molecular Blood Grouping Transfus Med Hemother 2013;40:454-458.

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A Novel <b><i>ABO</i></b> Gene Variant Leads to Discrepant Results in Forward/Reverse and Molecular Blood Grouping Transfus Med Hemother 2013;40:454-458 - DOI:10.1159/000356378 Fig. 1. Results from A forward and B reverse ABO typing of the patient using the gel technique. A and B antigens were clearly absent in antigen typing. Reverse typing failed to detect anti-A1 or anti-A2 isoagglutinins in the patient's serum. Anti-B isoagglutinins were normal (4+). C Antigen typing using further monoclonal reagents including an anti-AB monoclonal antibody led to very weak agglutination reaction detectable by a faint smear of erys in the gel. © 2013 S. Karger AG, Basel

A Novel <b><i>ABO</i></b> Gene Variant Leads to Discrepant Results in Forward/Reverse and Molecular Blood Grouping Transfus Med Hemother 2013;40:454-458 - DOI:10.1159/000356378 Fig. 2. Results from ABO genotyping using different PCR-SSP systems. A ABO-Type (BAG Healthcare) indicated genotype O1/O1; B ABO-SSP (Inno-Train Diagnostik GmbH) and C inhouse system both indicated genotype O1/A. The discrepancy resulted from the second reaction (nonO1) specific for the detection of 261G. The PCR products of internal controls are indicated by arrowheads. Note the weaker PCR band in the nonO1 reaction of the inhouse system. © 2013 S. Karger AG, Basel

A Novel <b><i>ABO</i></b> Gene Variant Leads to Discrepant Results in Forward/Reverse and Molecular Blood Grouping Transfus Med Hemother 2013;40:454-458 - DOI:10.1159/000356378 Fig. 3. Sequence analysis of ABO exon 6 in the patient's DNA. Heterozygosities were seen at position 248A>G (novel variant) and 261G>del (O1 allele). The dashed line indicates the binding region of the forward PCR-SSP primer specific for the O1 (261del) and the nonO1 alleles (261G). © 2013 S. Karger AG, Basel

A Novel <b><i>ABO</i></b> Gene Variant Leads to Discrepant Results in Forward/Reverse and Molecular Blood Grouping Transfus Med Hemother 2013;40:454-458 - DOI:10.1159/000356378 Fig. 4. Genotyping of the 248A>G mutation by PCR-SSP. A The 248G-specific reaction was positive (144 bp) in the patient sample (1) but negative in donors with regular ABO phenotype (2-5). B Representative result of screening for the 248A>G mutation in blood donors by PCR-SSP. For efficient screening we pooled 7 DNA samples and tested 260 pools. As a positive control (+) we pooled the patient DNA carrying the 248A>G mutation with 6 negative DNAs. The PCR products of internal controls are indicated by arrowheads. None of the pools were positive for the 248G allele (shown for pools 1-12). © 2013 S. Karger AG, Basel