Figure 1. A) IgE binding to L perenne proteins and processed peptides by western blot (2.3 µg of proteins and peptides have been loaded on SDS gel) . B)

Slides:

Advertisements

Similar presentations

Biochemistry Part 3 Lehningers Biochemistry. Amino Acid.

Advertisements

Supplemental Data for Two rings of negative charges in the cytosolic vestibule of type-1 ryanodine receptor modulate ion fluxes. by Le Xu, Ying Wang, Dirk.

Supplementary Figure 1. Importance of first neighbours in breast cancer, hepatocellular carcinoma and non-small cell lung cancer in the SignaLink network.

Natural latex, grass pollen, and weed pollen share IgE epitopes

B A P-AXL P-AXL AXL AXL P-MET P-MET MET MET P-Akt-473 P-Akt-473 AKT

From: Regulation of ADAM10 and ADAM17 by Sorafenib Inhibits Epithelial-to-Mesenchymal Transition in Epstein-Barr Virus–Infected Retinal Pigment Epithelial.

Fig. 4. Expressional profiles of apolipoproteins in HDL

From: Inhalational Anesthetics Disrupt Postsynaptic Density Protein-95, Drosophila Disc Large Tumor Suppressor, and Zonula Occludens-1 Domain Protein Interactions.

Blot, Blot, Western Baby Kristin B. Dupre June 30th, 2011.

Volume 145, Issue 2, Pages (August 2013)

Lolium perenne peptides for treatment of grass pollen allergy: A randomized, double- blind, placebo-controlled clinical trial Mohamed H. Shamji, PhD,

Soy Isoflavones Augment Radiation Effect by Inhibiting APE1/Ref-1 DNA Repair Activity in Non-small Cell Lung Cancer Vinita Singh-Gupta, PhD, Michael.

Wheat α-amylase inhibitor: A second route of allergic sensitization

Fig. 6. Sh-lantibiotics are commonly found on healthy human skin and synergize with a host AMP. Sh-lantibiotics are commonly found on healthy human skin.

The effects of dasatinib on IgE receptor–dependent activation and histamine release in human basophils by Michael Kneidinger, Uwe Schmidt, Uwe Rix, Karoline.

Volume 133, Issue 5, Pages (November 2007)

Development and characterization of a recombinant, hypoallergenic, peptide-based vaccine for grass pollen allergy Margarete Focke-Tejkl, PhD, Milena.

Volume 4, Issue 3, Pages (September 2006)

Natural latex, grass pollen, and weed pollen share IgE epitopes

Blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy Raphaela.

NG2-CreERT2 postnatal day 31

Molecular engineering of a therapeutic antibody for Blo t 5–induced allergic asthma J.H. Sherlynn Chan, BSc(Hons), Yen Leong Chua, MSc, Hong Yong Peh,

Induction of IgE antibodies with predefined specificity in rhesus monkeys with recombinant birch pollen allergens, Bet v 1 and Bet v 2 Fatima D. Ferreira,

Proteins Peptides DnaK Peptides + DnaK

Sabine Fischer, MSc,a, Monika Grote, PhD,b, B. Fahlbusch, PhD,c, W. D

Bullous Allergic Hypersensitivity to Bed Bug Bites Mediated by IgE against Salivary Nitrophorin Martin Leverkus, Ryan C. Jochim, Susanne Schäd, Eva-Bettina.

Birch pollen immunotherapy results in long-term loss of Bet v 1–specific TH2 responses, transient TR1 activation, and synthesis of IgE-blocking antibodies

Volume 28, Issue 3, Pages (September 2015)

a b MCF-7 TR2 MCF-7 TR2 (Fold change) MTT Assay , (Fold change)

Fig. 2. VEGF-C/VEGFR-3 signaling increases responsiveness of melanoma to immunotherapy. VEGF-C/VEGFR-3 signaling increases responsiveness of melanoma to.

Utz Fischer, Qing Liu, Gideon Dreyfuss Cell

The Function of Lysyl-tRNA Synthetase and Ap4A as Signaling Regulators of MITF Activity in FcϵRI-Activated Mast Cells Yu-Nee Lee, Hovav Nechushtan, Navah.

Sp1 Is Required for Glucose-Induced Transcriptional Regulation of Mouse Vesicular Glutamate Transporter 2 Gene Tao Li, Liqun Bai, Jing Li, Suzu Igarashi,

A Mechanism for Inhibiting the SUMO Pathway

Volume 110, Issue 11, Pages (June 2016)

Volume 127, Issue 1, Pages (July 2004)

Volume 25, Issue 2, Pages (February 2017)

Component-Resolved Diagnosis (CRD) of Type I Allergy with Recombinant Grass and Tree Pollen Allergens by Skin Testing Susanne Heiss, Rudolf Valenta

Mohamed H. Shamji, PhD, Janice A. Layhadi, MSc, Guy W

Regeneration of Human Dermis by a Multi-Headed Peptide

Allergen microarray detects high prevalence of asymptomatic IgE sensitizations to tropical pollen-derived carbohydrates Clarissa R. Cabauatan, PhD, Christian.

Major cat and dog allergens share IgE epitopes

“Auto-anti-IgE”: Naturally occurring IgG anti-IgE antibodies may inhibit allergen-induced basophil activation Yih-Chih Chan, PhD, Faruk Ramadani, PhD,

Glycolate Oxidase Is a Safe and Efficient Target for Substrate Reduction Therapy in a Mouse Model of Primary Hyperoxaluria Type I Cristina Martin-Higueras,

Functional Implications of the IL-6 Signaling Pathway in Keloid Pathogenesis Mohammad Ghazizadeh, Mamiko Tosa, Hajime Shimizu, Hiko Hyakusoku, Oichi Kawanami

Induced-Decay of Glycine Decarboxylase Transcripts as an Anticancer Therapeutic Strategy for Non-Small-Cell Lung Carcinoma Jing Lin, Jia Hui Jane Lee,

Role of IL-35 in sublingual allergen immunotherapy

Glucosamine sulfate modulates the levels of aggrecan and matrix metalloproteinase-3 synthesized by cultured human osteoarthritis articular chondrocytes

Volume 16, Issue 2, Pages (February 2009)

Marta Vilalta, Marjan Rafat, Amato J. Giaccia, Edward E. Graves

Induced-Decay of Glycine Decarboxylase Transcripts as an Anticancer Therapeutic Strategy for Non-Small-Cell Lung Carcinoma Jing Lin, Jia Hui Jane Lee,

Volume 25, Issue 2, Pages (February 2017)

An Electrophoretic Mobility Shift Assay Identifies a Mechanistically Unique Inhibitor of Protein Sumoylation Yeong Sang Kim, Katelyn Nagy, Samantha Keyser,

Volume 20, Issue 1, Pages (January 2012)

Western blotting analysis of purified cytoplasmic membranes.

Volume 11, Issue 4, Pages (February 2001)

John W. Haycock, Mark Wagner, Sheila Mac Neil

Fig. 2. Sera IgE reactivity patterns and the frequency of reactivity to raw and cooked shrimp extracts by Western blot. (A, C) All sera were diluted at.

A hypoallergenic cat vaccine based on Fel d 1–derived peptides fused to hepatitis B PreS Katarzyna Niespodziana, MSc, Margarete Focke-Tejkl, PhD, Birgit.

Western blot analysis of histone H1.X.

Leptin and amylin signaling.

Notch 1 Signaling Regulates Peripheral T Cell Activation

Volume 60, Issue 5, Pages (November 2001)

Lolium perenne peptides for treatment of grass pollen allergy: A randomized, double- blind, placebo-controlled clinical trial Mohamed H. Shamji, PhD,

Cucurbitacin I Inhibits Stat3 and Induces Apoptosis in Sézary Cells

Recognition of naturally processed HTLV-1 Tax protein derived from HAM patient's PBMC. A, HTLV-1 Tax protein content was evaluated by Western blot analysis.

Curcumin suppresses the expression of antiapoptotic proteins in multiple myeloma cells. Curcumin suppresses the expression of antiapoptotic proteins in.

Fig. 6. Sh-lantibiotics are commonly found on healthy human skin and synergize with a host AMP. Sh-lantibiotics are commonly found on healthy human skin.

Effect of SFN on the total activity and protein expression of HDACs in JB6 P+ cells. Effect of SFN on the total activity and protein expression of HDACs.

Presentation transcript:

Figure 1. A) IgE binding to L perenne proteins and processed peptides by western blot (2.3 µg of proteins and peptides have been loaded on SDS gel) . B) Capacity of the LP proteins and LPP to inhibit the binding of sIgE to pollen. C) Batch-to-batch reproducibility of LLP peptides. D-E) Ex-vivo basophil responsiveness to LP proteins, LPP, DnaK, LPP/DnaK in grass pollen allergics (n=12) and non-allergics (n=12). Wilcoxon rank test compared to corresponding concentration of LPP. (F-H) sIgE, sIgG4 and blocking antibody responses following of subcutaneous LLP, LLP peptides/DnaK or placebo. *<.05, **.01 and ***<.001. I-J) Number of well days and cumulative dose of LPP and LPP /DnaK.