Effects of a caspase-1 inhibitor and NLRP3 inhibitor on nontypeable Haemophilus influenzae (NTHi)-induced interleukin (IL)-1β production. a) Peripheral.

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Presentation transcript:

Effects of a caspase-1 inhibitor and NLRP3 inhibitor on nontypeable Haemophilus influenzae (NTHi)-induced interleukin (IL)-1β production. a) Peripheral blood mononuclear cells (PBMCs) and bronchoalveolar lavage (BAL) cells were cultured ex vivo for 24 h wit... Effects of a caspase-1 inhibitor and NLRP3 inhibitor on nontypeable Haemophilus influenzae (NTHi)-induced interleukin (IL)-1β production. a) Peripheral blood mononuclear cells (PBMCs) and bronchoalveolar lavage (BAL) cells were cultured ex vivo for 24 h with NTHi in the presence or absence of the caspase-1 inhibitor (C1I) Z-YVAD-FMK. IL-1β produced by healthy control (HC) PBMCs (n=20), protracted bacterial bronchitis (PBB) PBMCs (n=20), BAL macrophages (PBB BAL Mac) (n=9) and BAL neutrophils (PBB BAL Neut) (n=9). b) BAL macrophages (n=9) were cultured ex vivo for 24 h in the presence of NTHi and NLRP3 inhibitor MCC950. Data are presented as mean±sd. *: p<0.05; **: p<0.01; ***: p<0.001 by Wilcoxon matched-pairs signed rank test. Alice C-H. Chen et al. ERJ Open Res 2018;4:00130-2017 ©2018 by European Respiratory Society