Jie-Hong Pan, MD, PhD. a, Jes S. Lindholt, MD, PhD. b, Galina K

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Macrophage migration inhibitory factor is associated with aneurysmal expansion Jie-Hong Pan, MD, PhD*a, Jes S. Lindholt, MD, PhD*b, Galina K. Sukhova, PhDc, John A. Baugh, PhDd, Eskild W. Henneberg, MDe, Richard Bucala, MD, PhDd, Seamas C. Donnelly, MDd, Peter Libby, MDc, Christine Metz, PhDd, Guo-Ping Shi, DSca Journal of Vascular Surgery Volume 37, Issue 3, Pages 628-635 (March 2003) DOI: 10.1067/mva.2003.74 Copyright © 2003 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 1 MIF expression in normal arteries, atherosclerotic plaques, and aneurysmal lesions. MIF antigen largely colocalizes with CD68+ macrophages in both atheroma (n = 7) and AAA (n = 6). Normal arteries (n = 5) contain negligible MIF immunoreactivities. Goat nonimmune serum (NIS) negative controls for atheroma and AAA were shown in bottom two panels. Magnifications are indicated. Journal of Vascular Surgery 2003 37, 628-635DOI: (10.1067/mva.2003.74) Copyright © 2003 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 2 MIF expression in ECs and SMCs. In atheroma, MIF (right, red) is also expressed in CD31+ ECs (left, green) and α-actin+ SMCs (left, green). In AAAs, CD31+ ECs colocalize with immunoreactive MIF (see insets). In contrast, SMCs in AAAs express limited levels of MIF. Magnifications are indicated. Journal of Vascular Surgery 2003 37, 628-635DOI: (10.1067/mva.2003.74) Copyright © 2003 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions

Fig. 3 Scatter plots of correlation between MIF level and AAA expansion rate and initial AAA size in small AAAs. A, Correlation of MIF level with AAA expansion rate. Significant positive correlation of serum MIF level (S-Ln [MIF]; ng/mL) with AAA expansion rate (mm/y; r = 0.42; P =.001). B, Correlation of MIF level with initial AAA size. AAA initial sizes (mm) associate significantly with serum MIF level (S-Ln [MIF]; ng/mL; r = 0.41; P =.001). Journal of Vascular Surgery 2003 37, 628-635DOI: (10.1067/mva.2003.74) Copyright © 2003 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions