Ex vivo fluorescence confocal microscopy in conjunction with Mohs micrographic surgery for cutaneous squamous cell carcinoma  Caterina Longo, MD, PhD, Moira Ragazzi, MD, Stefano Gardini, MD, Simonetta Piana, MD, Elvira Moscarella, MD, Aimilios Lallas, MD, Margherita Raucci, ARNP, Giuseppe Argenziano, MD, Giovanni Pellacani, MD  Journal of the American Academy of Dermatology  Volume 73, Issue 2, Pages 321-322 (August 2015) DOI: 10.1016/j.jaad.2015.04.027 Copyright © 2015 American Academy of Dermatology, Inc. Terms and Conditions

Fig 1 A, Fluorescence confocal microscopy mosaic reveals a well-defined cutaneous squamous cell carcinoma silhouette with sharp demarcation and highly fluorescent areas (scale bar = 750 μm). B, Corresponding histopathologic images showing a well-differentiated tumor. C, Detail of the tumor showing keratin pearls (red arrows; scale bar = 150 μm). D, Histologic evaluation reveals the presence of several keratin pearls (red arrows). E, Fluorescence confocal microscopy overview of the tumor reveals a poorly defined tumor silhouette with a highly fluorescent area in the upper portion and lower fluorescence in the deepest part of the tissue corresponding to the infiltrative area (scale bar = 750 μm). F, Corresponding histopathologic image reveals a poorly differentiated tumor with irregular epithelial tongues. G, Few keratin pearls (red arrow) can be detected along with infiltrative cords of poorly differentiated keratinocytes (blue arrow; scale bar = 150 μm). H, Histopathologic image shows a poorly differentiated tumor with few keratin pearls (red arrow) and infiltrative cords (blue arrow). Journal of the American Academy of Dermatology 2015 73, 321-322DOI: (10.1016/j.jaad.2015.04.027) Copyright © 2015 American Academy of Dermatology, Inc. Terms and Conditions