Supplementary Figure 1 A B catalase p53 catalase MW(kDa) PIG3 MW(kDa)

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25 A hMLH1 B Acetylated H3 Enrichment SW480 RKO Position from +1 BBII ++-- SW480 BIBI ++-- RKO C hMLH1 GAPDH Ab D Methyl-H3-K4 enrichment Position.

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Presentation transcript:

Supplementary Figure 1 A B catalase p53 catalase MW(kDa) PIG3 MW(kDa) 72 55 43 34 26 95 130 170 PIG3 catalase MW(kDa) 72 55 43 34 1

Supplementary Figure 2 A B Vector PIG3 DCF fluorescence level Vector H1299 HCT116 p53-/- SW480 20 40 60 80 100 120 140 160  1.4    1.2 1.0 0.8 Catalase activity (U/ml) 0.6 0.4 0.2 0.0 SW480 H1299 HCT116 p53-/- PIG3 catalase -tubulin

Supplementary Figure 3 U2OS RKO HCT116 siRNA Control p53 Control p53 catalase p53R2 SESN1 SESN2 GPX1 ALDH4A1 SOD2 TIGAR Nrf-2 TP53INP1 GAPDH 3

Supplementary Figure 4 UV (10 J/m2) H2O2 (mM) HCT116 p53-/- HCT116 0 4 8 12 24 0 4 8 12 24 (hr) 0 0.05 0.1 1 2 0 0.05 0.1 1 2 p53 p53R2 catalase -tubulin UV (10 J/m2) H2O2 (mM) Saos-2 U2OS Saos-2 U2OS 0 4 8 12 24 0 4 8 12 24 (hr) 0 0.05 0.1 1 2 0 0.05 0.1 1 2 p53 p53R2 catalase -tubulin

Supplementary Figure 5 MW(kDa) 72 55 43 catalase p53R2 5

Supplementary Figure 6 A B ROS levels (%) 20 40 60 80 100 120 vector 20 40 60 80 100 120 vector wt R2 R4  n.s.  n.s. 250  200 150 Catalase activity (%) 100 50 vector wt R2 R4 6

Supplementary Figure 7 B A Control siRNA p53R2 siRNA 160 Control siRNA 140    120 n.s. 1.8 1.6 100 ROS levels (%) 1.4  80 n.s. 1.2 60 1.0 Catalase activity (U/ml) 0.8 40 0.6 20 0.4 0.2 U2OS RKO HCT116 0.0 U2OS RKO HCT116 p53R2 catalase -tubulin 7

Supplementary Figure 8 A B 2.5   n.s. n.s. n.s. 120 n.s. n.s.  Control vector Wild-type p53R2 Mutant p53R2 (V115L) Control vector Wild-type p53R2 Mutant p53R2 (V115L) 2.5   n.s. n.s. n.s. 120 n.s. n.s.    2.0 100  n.s. 1.5 80 Catalase activity (U/ml) 1.0 ROS levels (%) 60 40 0.5 20 0.0 U2OS RKO HCT116 p53R2 U2OS RKO HCT116 catalase -tubulin 8

Supplementary Figure 9 UT UV (10 J/m2) Sup P Sup P p53 PIG3 p53R2 Catalase β-actin PMP70

Supplementary Figure 10 catalase merge DAPI p53 UT p53R2 p53 UV (10 J/m2) p53R2

Supplementary Figure 11 A B U2OS (vector) U2OS (catalase) 200   180 35 160 30 140  25 120 ROS levels (%) Apoptosis (%) 100 20 80 15  60 10 40 20 5 8 24 h 24 48 h UV (40 J/m2) UV (40 J/m2)

Supplementary Figure 12 H1299 (vector) H1299 (catalase) 180  160 140 120 ROS levels (%) 100 80 60 40 20 Ad p53 48 h 12

Supplementary Figure 13 Untreated control UV (20 J/m2) 200  180 160 140 120 ROS levels (%) 100 80 60 40 20 Vector PIG3 HCT116 p53-/-