The isolation and characterization of a novel collagenolytic serine protease allergen (Der p 9) from the dust mite Dermatophagoides pteronyssinus Cecile.

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Presentation transcript:

The isolation and characterization of a novel collagenolytic serine protease allergen (Der p 9) from the dust mite Dermatophagoides pteronyssinus Cecile Kinga,b, Richard J. Simpsonc, Robert L. Moritzc, Gaven E. Reedc, Philip J. Thompsond, Geoffrey A. Stewarta,b Journal of Allergy and Clinical Immunology Volume 98, Issue 4, Pages 739-747 (October 1996) DOI: 10.1016/S0091-6749(96)70121-5 Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 1 Purification of Der p 9 by cation-exchange chromatography of SBTI affinity bound material. Bound material was eluted with distilled water adjusted to pH 11.0 with ammonia at point marked with the arrow. Protease activities were determined by using SA 2PLpNA (filled circles), SA 2PFpNA (open circles), and BApNA (filled squares). Hydrolysis of synthetic substrates was measured at 405 nm. Two fractions eluting at the pH ranges indicated by horizontal bars were pooled for further analysis. Journal of Allergy and Clinical Immunology 1996 98, 739-747DOI: (10.1016/S0091-6749(96)70121-5) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 2 SDS-PAGE analysis of isolated proteases. A, Lane 1 shows fraction 1 from Fig. 1 eluting between pH 4.5 and 9, and lane 2 shows fraction 2 eluting between pH 9 and 11. Lane 1 contains a mixture of the 30 kd trypsin (Der p 3) and the 28 kd protease (Der p 9) and a minor band at 14 kd. Lane 2 shows the 28 kd protease. B, Purified 25 kd Der p 6 chymotrypsin eluted by 0.3 mol/L phosphate buffer, pH 6.5, from a hydroxyapatite column (see Fig. 3). Journal of Allergy and Clinical Immunology 1996 98, 739-747DOI: (10.1016/S0091-6749(96)70121-5) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 3 Purification of the mite chymotrypsin by hydroxyapatite chromatography of copper-chelating Sepharose-bound material. Bound material was eluted with a phosphate-buffer gradient (dashed line) to 0.3 mol/L, followed by a 1 mol/L NaCl wash indicated by the arrow. Proteolytic activities of samples were determined by using SA 2PLpNA (filled circles) and SA 2PFpNA (open circles), and hydrolysis was measured at 405 nm. Journal of Allergy and Clinical Immunology 1996 98, 739-747DOI: (10.1016/S0091-6749(96)70121-5) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 4 Degradation of type III collagen (lane 1) from calf skin by clostridial collagenase (lane 2) and Der p 9 (lane 3) as judged by SDS-PAGE. Journal of Allergy and Clinical Immunology 1996 98, 739-747DOI: (10.1016/S0091-6749(96)70121-5) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 5 RAST analysis of individual mite allergens. Sera from 35 patients allergic to mites were tested for IgE reactivity to Der p 1, Der p 2, Der p 6, and Der p 9. Data for Der p 3 were obtained from the same panel but reported previously.2 Conditions for the assay were similar for both experiments. Individual patients’ scores are shown, and lines represent mean radiation bound added to twice the standard deviation for each disk type obtained by using sera from a non-mite-allergic panel (n = 12). Journal of Allergy and Clinical Immunology 1996 98, 739-747DOI: (10.1016/S0091-6749(96)70121-5) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 6 RAST-inhibition analysis showing allergenic cross-reactivity between Der p 9 and either Der p 6 or Der p 3 or Der p 9 itself as a control. Disks were coated with Der p 9, and RAST inhibition was performed with Der p 9 (filled circles), Der p 3 (filled triangles), and Der p 6 (filled squares). Journal of Allergy and Clinical Immunology 1996 98, 739-747DOI: (10.1016/S0091-6749(96)70121-5) Copyright © 1996 Mosby, Inc. Terms and Conditions