Comparative functional genomics of mammalian DNA methyltransferases

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Comparative functional genomics of mammalian DNA methyltransferases Nelida Rodriguez-Osorio, Hongfeng Wang, Jennifer Rupinski, Susan M. Bridges, Erdogan Memili Reproductive BioMedicine Online Volume 20, Issue 2, Pages 243-255 (February 2010) DOI: 10.1016/j.rbmo.2009.11.006 Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Dottup analysis of sequence similarities of the DNA methyltransferases DNMT1, DNMT2, DNMT3a, DNMT3b, and DNMT3L among mouse, human and bovine (window size: 10). A solid diagonal line indicates sequence similarity, breaks in the line indicate low similarity and a shift in the line indicates an insertion or a deletion. On each of the figures the species named first is the one on the y axis, and the second species is the one on the x axis. Reproductive BioMedicine Online 2010 20, 243-255DOI: (10.1016/j.rbmo.2009.11.006) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Phylogenetic trees of DNA methyltransferases (DNMT). Multiple sequence alignment was done using T-Coffee and the trees were generated using the Phylip maximum likelihood program with no molecular clock. Chicken was the outgroup for generating the trees. Bootstrap-values out of 250 replicates are shown for branches involving mammals. Reproductive BioMedicine Online 2010 20, 243-255DOI: (10.1016/j.rbmo.2009.11.006) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Multiple sequence alignment and functional domains of DNA methyltransferases (DNMT) from opossum, rat, mouse, dog, bovine, rhesus macaque, chimpanzee, and human. The visualization was produced using MSAVIS (Lindeman et al., 2007). Conserved domains (different colour tags at the bottom) were identified using the coding sequence tool at National Centre for Biotechnology Information. Positions in black represent identical amino acids for that column in all the species; positions in grey represent amino acids that differ. White indicates a gap. Reproductive BioMedicine Online 2010 20, 243-255DOI: (10.1016/j.rbmo.2009.11.006) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 4 Real time polymerase chain reaction for analysis of the DNA methyltransferases DNMT1, DNMT3a, and DNMT3b mRNA abundance in bovine oocytes and embryos. Bars represent relative expression values of 2-cell embryos, 8-cell embryos, and blastocysts (Blast) to the expression in metaphase II (MII) oocytes. Different letters represent statistically significant differences (P<0.05). Bars labelled (A and B) correspond to values not significantly different from both (A and B). Reproductive BioMedicine Online 2010 20, 243-255DOI: (10.1016/j.rbmo.2009.11.006) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions