Biomathematical Modeling: The Deletion of HMO1 and its Effect on Cold Shock Reaction in S. cerevisiae Lauren Magee & Lucia Ramirez Department of Mathematics.

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Presentation transcript:

Biomathematical Modeling: The Deletion of HMO1 and its Effect on Cold Shock Reaction in S. cerevisiae Lauren Magee & Lucia Ramirez Department of Mathematics and Biology Loyola Marymount University May 7, 2015

Significance Table from Anova Test WT HMO1 p < 0.05 2378 (38%) 903 (14.6%) p < 0.01 1527 (25%) 337 (5.4%) p < 0.001 860 (14%) 69 (1.1%) p < 0.0001 460 (7%) 18 (0.3%) B-H p < 0.05 228 (4%) 39 (0.6%) Bonferroni p < 0.05 8 (0.1%)

All Stem Profiles: wild type

Wt_Profile #37

Wt_Profile #40

Wt_Profile #49

Wt_Profile #29

Wt_Profile #25

Wt_Profile #10

Wt_Profile #6

Wt_Profile #26

Wt_Profile #0

HMO1_Profile #1 Only DNA binding evidence

HMO1_Profile #2 DNA binding and expression evidence

HMO1_Profile #13

HMO1_Profile #22

HMO1_Profile #38

HMO1_Profile #40

HMO1_Profile #43

HMO1_Profile #44

HMO1_Profile #49

*DNA binding plus expression evidence

Gene regulatory Network HMO1_profile43 *GRNsight software

Gene regulatory Network HMO1_profile43

Gene regulatory Network HMO1_profile43

Change in Expression after modifying fix_b? Fixed b = fix_b set to 1 Estimated b = fix_b set to 0

Gene regulatory Network HMO1_profile43

Estimated b: weighted network

Fixed b: weighted network

GRNmap model Fixed b Estimated b

GRNmap model Fixed b Estimated b

GRNmap model Fixed b Estimated b

GRNmap model Fixed b Estimated b

GRNmap model Fixed b Estimated b

GRNmap model Fixed b Estimated b

GRNmap model Fixed b Estimated b

GRNmap model Fixed b Estimated b

GRNmap model Fixed b Estimated b

GRNmap model Fixed b Estimated b

GRNmap model Fixed b Estimated b

GRNmap model Fixed b Estimated b

GRNmap model Fixed b Estimated b

Estimated vs. fixed b