Transient overexpression of FveCYP707A4a delayed fruit ripening.

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Transient overexpression of FveCYP707A4a delayed fruit ripening. Transient overexpression of FveCYP707A4a delayed fruit ripening. FveCYP707A4a was transiently overexpressed using an Agrobacterium infiltration method (65). Infiltration was performed at S7, and all analyses were conducted at 7 d after Agrobacterium infiltration. (A) Fruits overexpressing FveCYP707A4a remained unripe, while control fruits became ripe. (Scale bar: 1 cm.) (B) qRT-PCR analysis showed dramatic increase in FveCYP707A4a transcript levels in 35S::FveCYP707A4a fruits. Error bars represent SD of three independent replicates (15–20 fruits were used for each replicate). (C) ABA content in 35S::FveCYP707A4a fruits. Error bars represent SD of three independent replicates (10 fruits were used for each replicate). (D–F) The effect of transient expression of 35S::FveCYP707A4a on fruit firmness (D), transcript levels for three ripening-related genes (E), and NCED genes (F). Error bars represent SD of three independent replicates (15–20 fruits were used for each replicate). Letter in figure indicates significant differences between groups (P < 0.05, one-way ANOVA, Tukey’s HSD post hoc test). Xiong Liao et al. PNAS doi:10.1073/pnas.1812575115 ©2018 by National Academy of Sciences