The expression of anti‐viral genes is sustained for several days after IFN stimulation, along with increased levels of the STAT1, STAT2, and IRF9 proteins.

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The expression of anti‐viral genes is sustained for several days after IFN stimulation, along with increased levels of the STAT1, STAT2, and IRF9 proteins. The expression of anti‐viral genes is sustained for several days after IFN stimulation, along with increased levels of the STAT1, STAT2, and IRF9 proteins. (A) The expression of the anti‐viral genes IFI27, BST2, OAS2, and MX1 was analysed by real‐time PCR after stimulation of hTERT‐HME1 or BJ cells with IFNβ (3 IU/ml). The levels of gene expression were calculated semi‐quantitatively by using the ΔΔCt method (see Materials and methods). The data are represented as means of triplicate PCR analyses±standard deviations (s.d.). (B) hTERT‐HME1 or BJ cells were treated with IFNβ (50 IU/ml) and the levels of total IRF9 and STATs 1 and 2, or tyrosine‐phosphorylated STATs 1 and 2 (PY‐701‐STAT1 or PY‐690‐STAT2) were analysed by the western method. (C, D) AG14412 umbilical fibroblasts (C) and STAT1‐null fibroblasts transfected with the lentiviral vector containing wild‐type STAT1 (D) were used. Left, Cells were treated with IFNβ (50 IU/ml) and the levels of total IRF9, STAT1, and STAT2, or tyrosine‐phosphorylated STATs (PY‐701‐STAT1 or PY‐690‐STAT2) were analysed by the western method. Right, the expression of the IFI27, OAS2, and MX1 genes was analysed by real‐time PCR after stimulation with IFNβ (3 IU/ml). The levels of gene expression were calculated semi‐quantitatively by using the ΔΔCt method. The data are represented as means of triplicate PCR analyses±s.d.Source data for this figure is available on the online supplementary information page. HyeonJoo Cheon et al. EMBO J. 2013;32:2751-2763 © as stated in the article, figure or figure legend