Fig. S3 Human genome consensus coding sequence

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Fig. S3 Human genome consensus coding sequence ABCB8, ABCB10 28 exons Design primers for PCR amplification, HA analysis and direct sequencing of coding exons and intron-exon boundaries 8511 bp target sequences 24 primer pairs PCR amplification of 94 leukemia DNA samples 0.85 Mb total cancer sequences Direct sequencing When multiple SNPs are known in one exon Heteroduplex analysis Sequence samples with aberrant HA peaks (by universal M13 primers) 246 known SNPs observed 47 putative mutations observed 0 not present in remission samples/T cells/donors 68 frequency different from database 13 missense Validate in remission samples, cultured T cells or donor samples 13 present in T cells /donors /remission samples 21 new exonic changes 7 present in in remission samples/T cells/donors 8 silent 178 frequency same as database 1 undetermined to be somatic or germline 0 within 15 bases from exon-intron boundary 26 new intronic changes 26 not within 15 bases from exon-intron boundary