(A) gToW growth phenotypes occur in the absence of leucine, and red squares indicate a significant growth‐rate defect as compared with the empty plasmid.

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(A) gToW growth phenotypes occur in the absence of leucine, and red squares indicate a significant growth‐rate defect as compared with the empty plasmid control (Ø). (A) gToW growth phenotypes occur in the absence of leucine, and red squares indicate a significant growth‐rate defect as compared with the empty plasmid control (Ø). PBS2's growth rate could only be determined in the presence of leucine. (B) The severity of the growth defect increases with the level of leucine starvation and (C) they spread over different pathway functions. Graphs in (C) indicate growth with (black) or without (red) leucine. (D) Phenotypes caused by PBS2 and SSK1, unlike PTC2, are partially suppressed by the deletion of HOG1. (E) Overexpression of Pbs2p and Ssk1p causes dual phosphorylation of Hog1p, which after leucine limitation (12.5 mg/l), reaches levels comparable to those caused by osmotic stress (+) within 24 h. The empty plasmid control (pSBI40) remains similar to unstressed cells (−). Marcus Krantz et al. Mol Syst Biol 2009;5:281 © as stated in the article, figure or figure legend