3-Valve Pumping Sequence 4-Valve Pumping Sequence Instrumented Nanophysiometer for High Throughput Drug Delivery D. Michael Ackermann1, Jonathan Payne1, Hilary Samples1, James Wells1 Advisors: Franz Baudenbacher, Ph.D.2; Paul King, Ph.D.1 1 Department of Biomedical Engineering, Vanderbilt University, Nashville, TN 2 Department of Living State Physics, Vanderbilt University, Nashville, TN Objective Device Software Controller Design a nano-liter sized nanophysiometer suitable for cell culturing Implement on-chip peristaltic pumps for low volume flow through perfusion Incorporate thin film microelectrodes in the inflow and outflow channels for monitoring physiological parameters Develop a LabView user interface to manage valve sequencing Soft Lithography [1] S.R. Quake and A. Scherer, "From Micro to Nano Fabrication with Soft Materials", Science 290: 1536-40 (2000). [2] M.A. Unger, H.-P. Chou, T. Thorsen, A. Scherer, and S.R. Quake, "Monolithic Microfabricated Valves and Pumps by Multilayer Soft Lithography", Science 288: 113-116 (2000). Results 0.51 mm d Peristaltic Pumping Future Work Valve Flow Direction 1 2 3 Stage 3-Valve Pumping Sequence 4 Closed Valve Open Valve Flow Direction 1 2 3 Stage 4-Valve Pumping Sequence 4 Pump Sequencing (# of valves) Cells Electrodes pH Monitoring Use integrated thin film microelectrodes to monitor physiological parameters pH, glucose, etc. Electrodes coated with a substrate specific oxidase Catalyze reaction producing H2O2 H2O2 then detected Acknowledgments