Visual Tracking of Cell Boundaries and Geometries Andrew Gewitz, Eric Ho, Alvin Yates CS 223B Final Project March 17, 2008

Problem Statement Motivation Verification and building of models for cellular motion Broader Goal: Understand changes in signaling pathways over time Given a sequence of images, track cell movements through the given space http://www.eecs.berkeley.edu/~tomlin/CELL_SHAPE_MOVIES/Cad_GFP_19h_25h.mov

Approach Median Filtering Feature Identification - Cell Centroids Adaptive Thresholding Morphological Dilation and Erosion Motion Tracking / Correspondence Smooth Trajectory Model Minimization of Deviation from Set of Smoothest Trajectories

Centroid Correspondence and Trajectory

Future Work Parameter Optimization Alternatives Methods Automatic Feature Detection Changing Correspondence Metrics Contour Fitting Active Contour Methods Initialization Methods