FAM49B controls T cell activation by regulating cytoskeleton remodeling. FAM49B controls T cell activation by regulating cytoskeleton remodeling. (A) J.FAM49B.

Slides:

Advertisements

Similar presentations

by Miguel Vicente-Manzanares, Aranzazu Cruz-Adalia, Noa B

Advertisements

Posttranslational regulation of ETV5 and c-JUN in neurons.

Volume 21, Issue 5, Pages (November 2011)

The TCL1 oncoprotein inhibits activation-induced cell death by impairing PKCθ and ERK pathways by Gilles Despouy, Marjorie Joiner, Emilie Le Toriellec,

Expression of α5β1-integrin enhances EboV GP-mediated infection of GD25 cells. Expression of α5β1-integrin enhances EboV GP-mediated infection of GD25.

IP-MS identifies FAM49B interacting protein Rac.

TGF-β1 induces ILK activity in renal tubular epithelial cells.

Volume 19, Issue 2, Pages (July 2005)

PGAM5 dephosphorylates ASK1.

HDAC3, but not HDAC2 is in complex with tau protein.

The interaction of FAM49B and Rac.

Despite a relatively small amount of PI3Kβ being associated with activated PDGFRs, it generates a more substantial proportion of the PIP3 that accumulates.

Phosphatase activity of PGAM5 is required for activation of ASK1.

PGAM5 associates with and activates ASK1.

Themis acts to increase phosphorylation of Shp1.

Genome-wide CRISPR screen correctly identifies known regulators of proximal TCR signaling. Genome-wide CRISPR screen correctly identifies known regulators.

DOCK2 associates with CrkL and regulates Rac1 in human leukemia cell lines by Hiroshi Nishihara, Masae Maeda, Atsushi Oda, Masumi Tsuda, Hirofumi Sawa,

IMiD compounds affect CD34+ cell fate and maturation via CRBN-induced IKZF1 degradation by Shirong Li, Jing Fu, Hui Wang, Huihui Ma, Xiaoming Xu, Yong-Guang.

Absolute quantitation of class IA PI3K subunits and heterodimers in MEFs. (A) Immunoblot of endogenous p85α in lysates of MEFs (clones 1 or 2, genotypes.

Phosphorylation of CLEC-2 is dependent on lipid rafts, actin polymerization, secondary mediators, and Rac by Alice Y. Pollitt, Beata Grygielska, Bertrand.

Volume 28, Issue 3, Pages (September 2015)

Semaphorin-3A is expressed by tumor cells and alters T-cell signal transduction and function by Alfonso Catalano, Paola Caprari, Simona Moretti, Monica.

Subcellular localization of HIS-24::GFP in his-24−/− mutant background after B. thuringiensis (BT) infection. Subcellular localization of HIS-24::GFP in.

TCF19 is required for survival of NRAS‐mutant melanoma cells

NF1 downregulation activates MAPK pathway signaling.

Volume 51, Issue 6, Pages (September 2006)

Vimentin is dispensable for multiple B cell–intrinsic processes.

Volume 10, Issue 7, Pages (April 2000)

Volume 17, Issue 8, Pages (April 2007)

The role of SRC-C3G-RAP1 signaling in transformation induced by CRKL

Figure S3. Zhang et al. A B Myc-SIRT1 - + sir2a+/+ sir2a-/-

Association of endogenous cadherins with CDO and BOC

Daple is required for activation of Gαi3, Rac1, and AKT signals and in the antagonistic inhibition of β-catenin–dependent Wnt signals downstream of EGF/EGFR.

TRADD is recruited to the TLR4 signaling complex upon LPS stimulation.

TRAF4 is required for EGFR activation in response to EGF stimulation.

Human embryonic kidney (HEK293T) cells expressing mutated glucose transporter 1-flag gene (MUT-GLUT1-flag) uptake less glucose than their WT-GLUT1-flag.

Alterations to TNFR1 signaling and complex formation in the absence of TRADD. (A–C) ERK, JNK, and IκB. traddWT/WT and traddKO/KO MEFs (A) and BM-Macs (B)

Pi and FGF2 differentially activate the FGFR1-FRS2α-ERK pathway.

Volume 6, Issue 2, Pages (August 2009)

FGF/ERK inhibition accelerates neural fate conversion of EpiSCs.

High-affinity binding to the hypervariable region of the β1 I-like domain controls signaling to fibronectin fibrillogenesis. High-affinity binding to the.

MiR-200c/141 overexpression in LS-8 cells induces E-cad expression and cell-cell adhesion. miR-200c/141 overexpression in LS-8 cells induces E-cad expression.

Figure 4 DNM1 mutations affect protein levels and self-dimerization (A) HeLa cells were transfected with green fluorescent protein (GFP)-tagged mutant.

RXRa serves as a carrier for TR3 translocation initiated by 9-cis retinoic acid. RXRa serves as a carrier for TR3 translocation initiated by 9-cis retinoic.

High-affinity mutants of β3 integrin fail to stimulate RhoA activity and fibronectin fibrillogenesis. High-affinity mutants of β3 integrin fail to stimulate.

SUMOylation Promotes Nuclear Import and Stabilization of Polo-like Kinase 1 to Support Its Mitotic Function Donghua Wen, Jianguo Wu, Lei Wang, Zheng.

Expression of active Rho partially rescues Erk1/2 activation and peripheral FA phenotype in RIAM-knockdown cells. Expression of active Rho partially rescues.

Interaction of NM-GFPagg with G3BP and p62.

RhoA GTPase Regulates B Cell Receptor Signaling

eNOS regulated IR-induced NO generation and EGFR signal activation.

Volume 51, Issue 6, Pages (September 2006)

Vps36 interacts with Smo in the absence of Hh

Mutant MFN2 proteins have normal half-lives and are properly localized to mitochondria. Mutant MFN2 proteins have normal half-lives and are properly localized.

co-IP of LEDGF/p75 and MeCP2.

Variation in dUTPase expression in cell lines.

DGKζ and Cbl-b deficient T Cells have enhanced ERK1/2 and IκBα phosphorylation. DGKζ and Cbl-b deficient T Cells have enhanced ERK1/2 and IκBα phosphorylation.

DNA damage signaling regulates mutant p53 ubiquitination.

Volume 23, Issue 2, Pages (August 2005)

Geldanamycin-induced endocytosis and down-regulation of ErbB2 in cells expressing ErbB2 only. Geldanamycin-induced endocytosis and down-regulation of ErbB2.

Effects of visfatin on the cell proliferation and phosphorylation of ERK, Akt, and GSK-3β proteins in HCC cells. Effects of visfatin on the cell proliferation.

Ectopic expression of CA RSK1 mutant protects melanoma cells from PD98059-mediated apoptosis. Ectopic expression of CA RSK1 mutant protects melanoma cells.

PKCζ is tyrosine phosphorylated by EGF and contributes to EGF-induced activation of ERK in Mef cells. PKCζ is tyrosine phosphorylated by EGF and contributes.

Expression of dominant-negative RasN17 completely suppresses Ras activation in Rh1 cells. Expression of dominant-negative RasN17 completely suppresses.

Pirh2 represses p73-dependent transactivation.

A, expression of p53 downstream mediator p21.

BCG uptake by bladder cancer cells requires activated Pak1.

ZTL interacts with CO and regulates its stability.

Bid associates with TRAF6 in microglia and astrocytes, as shown by coimmunoprecipitation and PLA. A, Coimmunoprecipitation of Bid and TRAF6 in BV-2 cells.

MEK1/2-ERK1/2-p190RHOGAP and INK4a/ARF deficiency drive RHOA activation in NSCLC. A and B, G-LISA assay showing RHOA-GTP levels in H460, A549, and H838.

BRAF in-frame deletions mainly function as BRAF homodimers.

Presentation transcript:

FAM49B controls T cell activation by regulating cytoskeleton remodeling. FAM49B controls T cell activation by regulating cytoskeleton remodeling. (A) J.FAM49B cells stably expressing FAM49B or GFP were stimulated with anti-TCR. Cells were lysed at indicated times, Rac1–GTP pull-down assays were carried out using Pak-PBD beads. GTPγS and GDP-loaded lysates were used as controls. Total Rac1, β-actin, FAM49B and active Rac1 were detected by Western blot. (B) The samples in A were stimulated with C305 for times indicated. Phosphorylation of PAK was assessed by immunoblotting. (C) J.FAM49B cells were transduced with virus expressing empty vector (GFP), WT-FAM49B, or FAM49B (R161D) at an MOI of 0.5. Quantification of the F-actin level was assessed by flow cytometry. (D) J.FAM49B cells stably expressing WT-FAM49B, GFP, or FAM49B (R161D) were stimulated with anti-TCR for indicated time. Phosphorylation of PAK and ERK was assessed by immunoblotting. Wanjing Shang et al. PNAS 2018;115:17:E4051-E4060 ©2018 by National Academy of Sciences