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Presentation transcript:

Get out a scratch piece of paper. Biology 160 - warm up Get out a scratch piece of paper. Turn to a partner and together describe or draw a diagram of how PCR works.

Lab 06 - DNA Fingerprinting DNA fingerprinting can be used to determine whether or not two samples of genetic material are from a particular individual.

Short Tandem Repeat (STR) Analysis How do you prove that two samples of DNA come from the same person? Short tandem repeats (STRs) Are repetitive sequences of DNA that are repeated various times in the genome.

The Polymerase Chain Reaction (PCR) The polymerase chain reaction (PCR) is a technique by which any segment of DNA can be copied quickly and precisely. Through PCR, scientists can obtain enough DNA from even minute amounts of blood or other tissue to allow DNA fingerprinting. Copyright © 2007 Pearson Education Inc., publishing as Pearson Benjamin Cummings

Once a set of DNA fragments is prepared, The next step in STR analysis is to determine the lengths of these fragments.

Gel Electrophoresis Gel Electrophoresis Can be used to separate the DNA fragments obtained from different sources.

The DNA fragments are visualized as “bands” on the gel. The bands of different DNA samples can then be compared.