© 2013 Elsevier, Inc. All rights reserved. Chapter 19 Author: Kelly Elkins © 2013 Elsevier, Inc. All rights reserved.

© 2013 Elsevier, Inc. All rights reserved. Figure 19.1 Exponential amplification curves that show the results of forty cycles of RT-PCR using PowerPlex 16 (Promega) D7S820, D5S818, and Penta D primers depicted in Table 19.1. Curves of K562 DNA as detected by SYBR green I and a flat line for the negative control. © 2013 Elsevier, Inc. All rights reserved.

© 2013 Elsevier, Inc. All rights reserved. Figure 19.2 A. Melting curve plot from 50-95 °C for the three amplicons produced from K562 DNA (left image). B. First derivative plot of the melting curve from 50-95 °C showing the predominant peaks at 73.5 °C (D5S818), 76 °C (D7S820) and 78.5 °C (Penta D) for the amplicons produced from the K562 DNA (right image). © 2013 Elsevier, Inc. All rights reserved.

© 2013 Elsevier, Inc. All rights reserved. Figure 19.3 Sample 2% agarose gel of PCR of K562 DNA using PowerPlex16 primers D7S820, D5S818 and Penta D. Lanes 1 & 14 contains the Fisher BioReagents* exACTGene* DNA Ladders 2kb DNA Ladder (sizes indicated), Lanes 2-9 contain amplicons produced using 1000 pg, 500 pg, 100 pg, 50 pg, 10 pg, 5 pg, 1 pg, and 0 pg, respectively, and Lanes 10-13 are blank. © 2013 Elsevier, Inc. All rights reserved.