Lights on Iron-Sulfur Clusters Christopher Horst Lillig, Roland Lill  Chemistry & Biology  Volume 16, Issue 12, Pages 1213-1214 (December 2009) DOI: 10.1016/j.chembiol.2009.12.005 Copyright © 2009 Elsevier Ltd Terms and Conditions

Figure 1 Principle of the Fluorescence-Based Detection of Fe/S Cluster Formation in a Living Cell The glutathione (GSH)-dependent oxidoreductase Grx2 is fused independently to the N- and C-terminal fragments of a fluorescent protein (Venus). Upon formation of a bridging [2Fe-2S] cluster between two Grx2 monomers, the Venus fragments are located in immediate vicinity, increasing their chance to combine and generate the fluorescent conformation of Venus. The latter reaction requires the presence of molecular oxygen. The correlation between fluorescence emission and Fe/S cluster formation is demonstrated by the requirement of the cysteine desulfurase Nfs1 and the scaffold protein Isu1, both members of the mitochondrial ISC assembly machinery (Lill, 2009). Chemistry & Biology 2009 16, 1213-1214DOI: (10.1016/j.chembiol.2009.12.005) Copyright © 2009 Elsevier Ltd Terms and Conditions