PDE5A is expressed by podocytes.

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Presentation transcript:

PDE5A is expressed by podocytes. PDE5A is expressed by podocytes. RNA and protein were isolated from cultured podocytes and renal mouse cortex; subsequently, (A) PCR and (B) Western blot were performed to determine PDE5A expression in these samples. Glomerular PDE5A expression was confirmed by immunofluorescence staining of PDE5A. (C) Importantly, costaining with synaptopodin showed PDE5A expression in the podocytes; merge is a higher magnification of single-channel images. AB, antibody; NTC, no template control. Ramon Sonneveld et al. JASN 2017;28:1491-1505 ©2017 by American Society of Nephrology