MAGEA3 Expression in Cutaneous Squamous Cell Carcinoma Is Associated with Advanced Tumor Stage and Poor Prognosis Melody Abikhair, Nazanin Roudiani,

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MAGEA3 Expression in Cutaneous Squamous Cell Carcinoma Is Associated with Advanced Tumor Stage and Poor Prognosis Melody Abikhair, Nazanin Roudiani, Hiroshi Mitsui, James G. Krueger, Anna Pavlick, James Lee, Jean-Philippe Therrien, Shane A. Meehan, Diane Felsen, John A. Carucci Journal of Investigative Dermatology Volume 137, Issue 3, Pages 775-778 (March 2017) DOI: 10.1016/j.jid.2016.10.036 Copyright © 2016 The Authors Terms and Conditions

Figure 1 MAGEA3 is more highly expressed in invasive SCC nests compared with AK or SCC in situ. (a) Global gene expression analysis in laser captured AK and SCC regions compared with normal epidermis identified eight known CT antigens. The numbers highlighted in red and blue refer to significant up- and down-regulation, respectively. Fold change greater than 3.0 and false discovery rate smaller than 0.05 were considered differentially expressed. (b) Quantitative real-time polymerase chain reaction confirmed greater MAGEA3 expression in invasive SCC samples compared with AK or SCC in situ. (c) Prospectively obtained fresh tissue showed MAGEA3 was most highly expressed in invasive SCC. All scale bars = 100 μm. AK, actinic keratosis; CT, cancer testis; IHC, immunohistochemistry; NML, normal; SCC, squamous cell carcinoma; SCC 1 and 2, representative IHC from two invasive SCC samples. Journal of Investigative Dermatology 2017 137, 775-778DOI: (10.1016/j.jid.2016.10.036) Copyright © 2016 The Authors Terms and Conditions

Figure 2 MAGEA3 expression on NanoString analysis and IHC is correlated with advanced tumor stage and poor clinical outcomes. (a) Heatmap displaying MAGEA family gene expression from FFPE samples ordered by increasing BWH staging. MAGEA3 up-regulation is present in the highest-grade tumors. (b) MAGEA3 positivity on IHC of FFPE samples was graded from negative to ++++, with the most highly expressing tumors shown. (c) Double label IHC showed that MAGEA3-positive cells (brown) expressed pan-keratin and Ki67 (pink). All scale bars = 100 μm. BWH, Brigham and Women’s Hospital; FFPE, formalin-fixed paraffin-embedded; IHC, immunohistochemistry; PanK, pan-keratin. Journal of Investigative Dermatology 2017 137, 775-778DOI: (10.1016/j.jid.2016.10.036) Copyright © 2016 The Authors Terms and Conditions