Whole genome deoxyribonucleic acid microarray analysis of gene expression in ectopic versus eutopic endometrium  Kathleen M. Eyster, Ph.D., Olga Klinkova, M.D., Vanessa Kennedy, B.S., Keith A. Hansen, M.D.  Fertility and Sterility  Volume 88, Issue 6, Pages 1505-1533 (December 2007) DOI: 10.1016/j.fertnstert.2007.01.056 Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Differential expression of PLA2 IIA, PLA2 V, and fatty acid–binding protein 4 in eutopic versus ectopic endometrium. (A) Data from DNA microarray analysis. (B) Data from real-time reverse transcription–PCR (RT-PCR). (C) Amplification plot from real-time RT-PCR analysis of PLA2 IIA expression. Curve a represents PLA2 IIA expression in ectopic endometrium; curve b represents PLA2 IIA expression in eutopic endometrium. (D) Immunoblot analysis of PLA2 IIA in eutopic (lanes 1, 3, and 5) and ectopic (lanes 2, 4, and 6) endometrium. (E) and (F) Data from DNA microarray analysis and real-time RT-PCR, respectively, for PLA2 V. (G) and (H) DNA microarray data and real-time RT-PCR, respectively, for fatty acid–binding protein 4. Statistical significance for the DNA microarray data in A, E, and G was determined by t-test with use of GeneSpring software, P<.05. Differential expression for real-time RT-PCR data in panels B, F, and H was determined by t-test (P<.05) with use of GraphPad Prism. ∗Significant differences in A, B, and E through H. Fertility and Sterility 2007 88, 1505-1533DOI: (10.1016/j.fertnstert.2007.01.056) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Differential expression of glypican 6, prostacyclin synthase, leukotriene C4 synthase, and phospholipid scramblase 4 in eutopic versus ectopic endometrium. (A, C, E, and G) DNA microarray data and (B, D, F, and H) real-time reverse transcription–PCR data (RT-PCR) for four genes. Differential expression of DNA microarray data in A, C, E, and G was determined by t-test with use of GeneSpring software (P<.05). Differential expression for real-time RT-PCR in B, D, F, and H was determined by t-test (P<.05) with use of GraphPad Prism. ∗Significant differences in all panels. Fertility and Sterility 2007 88, 1505-1533DOI: (10.1016/j.fertnstert.2007.01.056) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Differential expression of complement component 7, haptoglobin, claudin 11, and uridine diphosphate glycosyltransferase 8 in eutopic versus ectopic endometrium. (A, C, E, and G) DNA microarray data and (B, D, F, and H) real-time reverse transcription–PCR (RT-PCR) data for the four genes. Differential expression of DNA microarray data in A, C, E, and G was determined by t-test with use of GeneSpring software (P<.05). Differential expression for real-time RT-PCR data in B, D, F, and H was determined by t-test (P<.05) with use of GraphPad Prism. ∗Significant differences in all panels. Fertility and Sterility 2007 88, 1505-1533DOI: (10.1016/j.fertnstert.2007.01.056) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions