الكميات المطلوبة لتحضير 50 ميكروليتر الحجم الكلي للتفاعل هي:

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الكميات المطلوبة لتحضير 50 ميكروليتر الحجم الكلي للتفاعل هي: الكميات المطلوبة لتحضير 50 ميكروليتر الحجم الكلي للتفاعل هي: Components Stock concentration Final concentration Template 125 ng/μL 7.5 ng/μL Primer F 200 ng/μL 4.0 ng/μL Primer R dNTP mix 2.0 mM each 0.2 mM PCR buffer 10 X 1 X MgCl2 25 mM 1.5 mM Taq polymerase 5 U/μL 0.05 U/μL H2O

الكميات المطلوبة لتحضير 20 ميكروليتر الحجم الكلي للتفاعل هي: الكميات المطلوبة لتحضير 20 ميكروليتر الحجم الكلي للتفاعل هي: Components Stock concentration Final concentration Template 125 ng/μL 7.5 ng/μL Primer F 200 ng/μL 4.0 ng/μL Primer R dNTP mix 2.0 mM each 200 μM PCR buffer 10 X 1 X MgCl2 25 mM 1500 μM Taq polymerase 5 U/μL 0.05 U/μL H2O

ما هو الحجم المطلوب إضافته إلى برايمر وزنه 23 نانو مول ليكون تركيزه النهائي 100 ميكرو مولر