Lab 3 streptococci.

Slides:

Advertisements

Similar presentations

Lab 6. Gram staining was originally developed in 1844 by Christian Gram Because most bacteria have one of these two types of cell walls, we can use this.

Advertisements

Differential medium. Differential medium helps us to differentiate one group of bacteria from another. Blood agar – differentiate hemolytic bacteria from.

Streptococcaceae II Jeanne Filbey MT(ASCP)

Lab. No Microscopical Appearance:(Gram’s Stain) Gram’s +ve Cocci Irregular ClustersChains or PairsTetrads Staphylococci MicrococciStreptococci.

Lab 14 Goals and Objectives: Exercise 69: Staphylococci Identification Read and record results Exercise 70: Streptococci & Enterococci Identification Read.

Isolation and Identification of Gram Positive Cocci

Medical bacteriology:

STREPTOCOCCUS. SpeciesLancefield GroupTypical HemolysisDiagnostic Features Str. pyogenesABetaBacitracin-sensitive Str. agalactiaeBBetaBacitracin-resistant;

226 PHT Lab#2 Staining techniques

Staph and Strep.

Lab 23 Respiratory Microorganisms. Objectives Interpret hemolysis results Classify various Streptococcus species.

Review Lab Exercises Exercise 8 zWhat is the purpose of this exercise? zWhat is a capsule? zWhat is the capsule made of? zWhat is the importance.

Introduction to bacteria

Streptococcus Basmah almaarik Lab # 6.

PHT 416 Lab 7. Steps Microscopic Morphology Growth Biochemical Tests Nutrient agar Blood agar Mannitol Salt Agar MacConkey’s agar.

Diagnosis of streptococci Compiled by Thamer Hamdan Compiled by Thamer Hamdan M.Sc. Clinical Microbiology and Immunology M.Sc. Clinical Microbiology and.

Streptococcus Gram+ cocci In chains.

Streptococci. Introduction Pyogenic pathogens - nonmotile, catalase negative, Gram positive cocci in chains.

Gram Staining Method Biology 2 Mr. Beyer. Individual Supplies Microscope Slide Clothes Pin Inoculating Loop or Wood Splint Paper Towels.

Identification of Staphylococci and Streptococci

Nora Al-Kubaisi Gram’s +ve Cocci Irregular Clusters Tetrads Chains or Pairs Staphylococci Micrococci Streptococci.

PHT 313 Lab (3) Streptococci. Staphylococci Streptococci Enterococci NeisseriaCorynbacterium Clostridum Bacillus Enterobacteriaceae Pseudomonas. Bacteria.

LAB 7: RESPIRATORY SYSTEM. RESPIRATORY SYSTEM: UPPER AND LOWER.

General Microbiology Laboratory Isolation and Identification of Gram Positive Cocci.

Observing Microorganisms Through a Microscope

StainsStains. Cheek Cells Without Stain Cheek Cells with Simple Stain (Methylene Blue)

Gram staining. Types of staining Simple –Bacteria stained to contrast environment Differential –Bacteria differing chemically react differently to staining.

Lab. No. 3. Microscopical Appearance:(Gram’s Stain) Microscopical Appearance:(Gram’s Stain) Gram’s +ve Cocci Irregular ClustersChains or PairsTetrads.

Experiment 2 Gram Stain and Acid-fast Stain.  Staining - simple stain (only one dye) - differential stain (more than one dye) G+G+ G-G- Gram stain Acid-fast.

Biology 11 Microbiology Gram staining. What is Gram Staining? Basic classification of bacteria that is based on the cell wall structure. There are 2 main.

THE GRAM STAIN. The gram stain is the most widely used staining procedure in bacteriology It is called a differential stain it differentiates between.

StainsStains. Negative Stain Cheek Cells Without Stain.

Lab 14 Goals and Objectives: Exercise 69: Staphylococci Identification Read and record results Exercise 70: Streptococci & Enterococci Identification Read.

Gram Staining. Aseptic Technique Sterilize Everything – Use bunsen burner or Bacti-Cinerator.

Bacterial Identification

Streptococci M.O : Disease S.agalactia,S.dysagalactia : mastitis in cattle,sheep S.equi subsp. Equi : Strangles, Suppurative conditions (horse) S.zooepidemicus.

Streptococcaceae family Classification - Includes 7 genera: –Streptococcus – a major cause of human infections –Enterococcus – frequent cause of human.

Exercise 39: Oxidation and Fermentation Tests (Catalase)

Differential staining

PHT313 Lab. No. 3.

GRAM and ACID FAST STAINS

PRACTICAL GRAM STAINING

بسم الله الرحمن الرحيم 140 Micro Lab 7: Gram stain.

Lecturer Ishraq Hasan Elewi.

Gram Staining: Principle, Procedure and Results MSc. Sarah Ahmed

Gram’s stain Acid fast stain Spore stain Hanging drop technique

Bacterial Cell Walls.

PRACTICAL GRAM STAINING

THE GRAM STAIN.

2 loops each organism Wax Pencil mixed together

PRACTICAL GRAM STAINING

Staphylococcus Streptococcus

Streptococci Basmah almaarik

Week 5 B Exp 10: Gram Stain Gram Stain: Gram + SE, SA

Practical 2 Gram staining.

Observing Microorganisms Through a Microscope

Cocci. Cocci Bacilli Spirochetes Staphylococcus Arrangement.

Lab 3 Staining.

طريقة تخطيط الاطباق Streak Plate Method

II- Streptococci Characters of Streptococci Gram positive cocci

How to look at bacteria &

Streptococcus pneumoniae

Medical Microbiology Lab. 3 By Assistant lecturer Zainab farooq shafeeq.

Presentation transcript:

Lab 3 streptococci

Streptococci Gram + cocci Chains in arrangement Catalase negative

Types of hemolysis : Blood agar

Alpha hemolysis Partial hemolysis of RBCs Mainly S.pneumoniae & S. viridans To differentiate: optochin disc S.pneumoniae is sensitive while S. viridans is resistant.

Beta- hemolysis Complete Hemolysis of RBCs Mainly GAS (Group A streptococci ) & GBS (Group B streptococci ) To differentiate: bacitracin test GAS is sensitive while GBS is resistant.

Gamma – hemolysis No hemolysis Mainly Enterococci : E. faecium & E. feacalis Can grow at 6.5 % salt NACL Can hydrolyze esculin in 40% bile ( bile esculin) BILE inhibits gram + bacteria except enterococci & group D streptococci

summary

Gram stain 1- primary stain: ( crystal violet ) 1 minute 2- mordant : ( iodine ) 1 minute 3- decolorizer : ( alcohol or acetone ) 1-2 seconds 4- counterstain : ( safranin ) 1 minute