Stains.

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Ex. 5: Acid-Fast and Endospore Stains
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Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case M I C.
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Presentation transcript:

Stains

Cheek Cells Without Stain

Cheek Cells with Simple Stain (Methylene Blue)

Simple Stain: Negative Stain

Differential Stains: Gram Stain Color of Gram + Gram – Primary stain: Crystal violet Purple Mordant: Iodine Decolorizing agent: Alcohol-acetone Colorless Counterstain: Safranin Pink

Gram Positive and Gram Negative Bacteria

Prokaryotic Cell Drawing

Outer membrane Peptidoglycan GRAM NEGATIVE GRAM POSITIVE

Capsule Stain

Capsule Stain

Figure 3.12a-c

Acid-Fast and Non Acid-Fast

Endospore Stain: Ziehl-Neelsen

Figure 3.12a-c

Endospore Stain

Stain Comparisons G+ (PURPLE) G – (PINK) ACID FAST PRIMARY STAIN ENDOSPORE STAIN PRIMARY STAIN Crystal violet Carbol Fuschia Malachite green MORDANT Iodine Heat DECOLORIZER ETOH or acetone: Cell is purple Cell is clear Acid alcohol Water COUNTER-STAIN Safranin: Cell is pink Methylene Blue Spores are

Flagella Stain

Flagella Stain

Figure 3.12a-c

Names for flagella arrangement Monotrichous Lophotrichous Amphitrichous Peritrichous

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