Fecal microbiota transplantation: A ‘How-To’ guide for nurses Sharyn Leis, RN, Thomas Julius Borody, MD, PhD, Chongnan Jiang, MD, Jordana Campbell, BSc  Collegian  Volume 22, Issue 4, Pages 445-451 (December 2015) DOI: 10.1016/j.colegn.2014.08.002 Copyright © 2014 Australian College of Nursing Ltd. Terms and Conditions

Figure 1 Blood and stool testing should be performed for both the donor and the intended FMT recipient. Testing should include infectious disease screening, including HIV and Hepatitis A, B, C, as well as general measures of health, including standard hematology and biochemistry. A 3-day stool test should be used to ensure any infections with difficult-to-detect pathogens are not overlooked. Collegian 2015 22, 445-451DOI: (10.1016/j.colegn.2014.08.002) Copyright © 2014 Australian College of Nursing Ltd. Terms and Conditions

Figure 2 Donor collection kit, consisting of stool collection ‘hat’, clear plastic containers to be labeled with the donor's information and opaque plastic delivery bags. Collegian 2015 22, 445-451DOI: (10.1016/j.colegn.2014.08.002) Copyright © 2014 Australian College of Nursing Ltd. Terms and Conditions

Figure 3 FMT syringes ready for infusion via colonoscope. Syringes are sealed with stoppers following preparation and are covered and delivered to the endoscopy suite immediately prior to the colonoscopy procedure. Collegian 2015 22, 445-451DOI: (10.1016/j.colegn.2014.08.002) Copyright © 2014 Australian College of Nursing Ltd. Terms and Conditions

Figure 4 Contents of the FMT syringes are infused one at a time via the biopsy channel of the colonoscope. A Luer-Lock attachment on the biopsy channel will facilitate incident-free infusion. Collegian 2015 22, 445-451DOI: (10.1016/j.colegn.2014.08.002) Copyright © 2014 Australian College of Nursing Ltd. Terms and Conditions

Figure 5 Enema bag with pediatric enema tip (blue) attached. Adult enema tips (orange) may also be used. A white enema bag clamp is visible near the point of attachment of the enema tip. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Collegian 2015 22, 445-451DOI: (10.1016/j.colegn.2014.08.002) Copyright © 2014 Australian College of Nursing Ltd. Terms and Conditions

Figure 6 Purpose-built inclined beds for the infusion of FMT via rectal enema. Collegian 2015 22, 445-451DOI: (10.1016/j.colegn.2014.08.002) Copyright © 2014 Australian College of Nursing Ltd. Terms and Conditions