Identification and Characterization of Optimal Gene Expression Markers for Detection of Breast Cancer Metastasis John Backus, Todd Laughlin, Yixin Wang,

Slides:

Advertisements

Similar presentations

Functional Analysis of the Neurofibromatosis Type 2 Protein by Means of Disease- Causing Point Mutations Renee P. Stokowski, David R. Cox The American.

Advertisements

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.

Nine Novel Germline Gene Variants in the RET Proto-Oncogene Identified in Twelve Unrelated Cases Syed A. Ahmed, Karen Snow-Bailey, W. Edward Highsmith,

A Multiplex qPCR Gene Dosage Assay for Rapid Genotyping and Large-Scale Population Screening for Deletional α-Thalassemia Wanjun Zhou, Ge Wang, Xuefeng.

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,

Telomerase Activity and Expression of Telomerase RNA Component and Telomerase Catalytic Subunit Gene in Cervical Cancer Kenji Nakano, Elizabeth Watney,

Molecular Testing Guideline for Selection of Lung Cancer Patients for EGFR and ALK Tyrosine Kinase Inhibitors Neal I. Lindeman, Philip T. Cagle, Mary Beth.

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.

Quantification of Parvovirus B19 DNA Using COBAS AmpliPrep Automated Sample Preparation and LightCycler Real-Time PCR Stefan Schorling, Gunnar Schalasta,

Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.

Accurate Molecular Characterization of Formalin-Fixed, Paraffin-Embedded Tissues by microRNA Expression Profiling Anna E. Szafranska, Timothy S. Davison,

Transgenic Mice Overexpressing BMP4 Develop a Fibrodysplasia Ossificans Progressiva (FOP)-Like Phenotype Lixin Kan, Min Hu, William A. Gomes, John A. Kessler.

Duplex Ratio Tests as Diagnostic Biomarkers in Malignant Melanoma

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis Philippe Szankasi, David.

Statistical Considerations for Immunohistochemistry Panel Development after Gene Expression Profiling of Human Cancers Rebecca A. Betensky, Catherine.

Droplet Digital PCR for Absolute Quantification of EML4-ALK Gene Rearrangement in Lung Adenocarcinoma Qiushi Wang, Xin Yang, Yong He, Qiang Ma, Li Lin,

Lunx Is a Superior Molecular Marker for Detection of Non-Small Lung Cell Cancer in Peripheral Blood Michael Mitas, Loretta Hoover, Gerard Silvestri,

A Quantitative Reverse Transcriptase-Polymerase Chain Reaction Assay to Identify Metastatic Carcinoma Tissue of Origin Dimitri Talantov, Jonathan Baden,

Laboratory Guidelines for Detection, Interpretation, and Reporting of Maternal Cell Contamination in Prenatal Analyses Narasimhan Nagan, Nicole E. Faulkner,

Eligibility Criteria and Genetic Testing Results from a High-Risk Cohort for Hereditary Breast and Ovarian Cancer Syndrome in Southeastern Ontario Ricardo.

A Long Noncoding RNA Signature That Predicts Pathological Complete Remission Rate Sensitively in Neoadjuvant Treatment of Breast Cancer Gen Wang, Xiaosong.

Molecular Genetics of Pediatric Soft Tissue Tumors

A Quantitative Reverse Transcription-PCR Assay for Rapid, Automated Analysis of Breast Cancer Sentinel Lymph Nodes Steven J. Hughes, Liqiang Xi, William.

A DNA Real-Time Quantitative PCR Method Suitable for Routine Monitoring of Low Levels of Minimal Residual Disease in Chronic Myeloid Leukemia Paul A.

Mutation Screening in Juvenile Polyposis Syndrome

Detection of Cytomegalovirus in Whole Blood Using Three Different Real-Time PCR Chemistries Erica Vincent, Zhengming Gu, Markus Morgenstern, Candace.

Detection of Microsatellite Instability in Colorectal Cancer Using an Alternative Multiplex Assay of Quasi-Monomorphic Mononucleotide Markers Vanessa.

Development and Evaluation of a Multiplex Real-Time Polymerase Chain Reaction Procedure to Clinically Type Prevalent Salmonella enterica Serovars Nélida.

Joanna Wang, Chetan Bettegowda The Journal of Molecular Diagnostics

Increased Sensitivity of the Roche COBAS AMPLICOR HCV Test, Version 2

Maxim B. Freidin, Neesa Bhudia, Eric Lim, Andrew G

Novel Molecular Method for Detection of Bovine-Specific Central Nervous System Tissues as Bovine Spongiform Encephalopathy Risk Material in Meat and Meat.

Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar

Quantification of Melanoma mRNA Markers in Sentinel Nodes

Molecular Therapy Volume 20, Pages S261-S262 (May 2012) DOI: /S (16)

Rapid Real-Time PCR Assays for Detection of Klebsiella pneumoniae with the rmpA or magA Genes Associated with the Hypermucoviscosity Phenotype Laurie.

Influenza A Subtyping The Journal of Molecular Diagnostics

Patrick R. Murray The Journal of Molecular Diagnostics

William L. Gerald, M.D., Ph.D, 1954–2008

Yuqiu Jiang, Graham Casey, Ian C

Detection and Typing of Viruses Using Broadly Sensitive Cocktail-PCR and Mass Spectrometric Cataloging Charul Gijavanekar, Rafal Drabek, Mithil Soni,

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis Philippe Szankasi, David.

Development of an NPM1/MLF1 D-FISH Probe Set for the Detection of t(3;5)(q25;q35) Identified in Patients with Acute Myeloid Leukemia Umut Aypar, Ryan.

Expression of Two Breast-Specific Molecules in the Lung

Gene Expression Screening of Salivary Gland Neoplasms

Prognostic Gene Expression Signatures Can Be Measured in Tissues Collected in RNAlater Preservative Dondapati Chowdary, Jessica Lathrop, Joanne Skelton,

Raymond R. Tubbs, James Pettay, Pat Roche, Mark H

Comparison of KRAS Mutation Analysis and FISH for Detecting Pancreatobiliary Tract Cancer in Cytology Specimens Collected During Endoscopic Retrograde.

Duplex Ratio Tests as Diagnostic Biomarkers in Malignant Melanoma

The Molecular Pathology of Primary Immunodeficiencies

Susan M. Farrington, Juili Lin-Goerke, Jessica Ling, Yute Wang, John D

Databases for Clinical Research

Report of New Haplotype for ABCC2 Gene

Validation of a Gene Expression Test for Mesothelioma Prognosis in Formalin-Fixed Paraffin-Embedded Tissues Assunta De Rienzo, Robert W. Cook, Jeff Wilkinson,

Testing for Maternal Cell Contamination in Prenatal Samples

Identification of MGB1 as a Marker in the Differential Diagnosis of Lung Tumors in Patients with a History of Breast Cancer by Analysis of Publicly Available.

The Detection of t(14;18) in Archival Lymph Nodes

Intratumoral Heterogeneity of MicroRNA Expression in Breast Cancer

A Sample Extraction Method for Faster, More Sensitive PCR-Based Detection of Pathogens in Blood Culture John F. Regan, Manohar R. Furtado, Maxim G. Brevnov,

Rapid Polymerase Chain Reaction-Based Detection of Epidermal Growth Factor Receptor Gene Mutations in Lung Adenocarcinomas Qiulu Pan, William Pao, Marc.

Use of Pyrosequencing of 16S rRNA Fragments to Differentiate between Bacteria Responsible for Neonatal Sepsis Jeanne A. Jordan, Allyson R. Butchko, Mary.

Karen Snow-Bailey, Ph.D., 1961–2006

Custom Design of a GeXP Multiplexed Assay Used to Assess Expression Profiles of Inflammatory Gene Targets in Normal Colon, Polyp, and Tumor Tissue Janice.

Journal of Investigative Dermatology

Xiangfeng Cui, Helen Feiner, Honghua Li

Improved Detection of the KIT D816V Mutation in Patients with Systemic Mastocytosis Using a Quantitative and Highly Sensitive Real-Time qPCR Assay Thomas.

Efficient Identification of miRNAs for Classification of Tumor Origin

Angiotensin I-Converting Enzyme

Maureen J. O'Sullivan, Peter A. Humphrey, Louis P. Dehner, John D

Monique A. Johnson, Marvin J. Yoshitomi, C. Sue Richards

Presentation transcript:

Identification and Characterization of Optimal Gene Expression Markers for Detection of Breast Cancer Metastasis John Backus, Todd Laughlin, Yixin Wang, Robert Belly, Robert White, Jon Baden, C. Justus Min, Ann Mannie, Lorraine Tafra, David Atkins, Kathryn M. Verbanac The Journal of Molecular Diagnostics Volume 7, Issue 3, Pages 327-336 (August 2005) DOI: 10.1016/S1525-1578(10)60561-2 Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Marker selection process. Identification of breast-specific markers and/or breast cancer-status markers was carried out using a series of four filters: 1) expression in WBCs, 2) expression level in breast cancer tissues, 3) expression level in normal/benign breast tissues, and 4) expression in other epithelial cancers. Markers had to pass the first two criteria and either of the last two criteria to be selected. The Journal of Molecular Diagnostics 2005 7, 327-336DOI: (10.1016/S1525-1578(10)60561-2) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Sensitivity of markers in lymph nodes. Individual marker sensitivity was assessed in lymph node tissues by RT-PCR. A specificity of 94% was used to identify individual markers that offered optimal performance for detection of clinically actionable metastases. The Journal of Molecular Diagnostics 2005 7, 327-336DOI: (10.1016/S1525-1578(10)60561-2) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Sensitivity of marker combinations in lymph nodes. Two-marker and three-marker combinations were assessed in lymph node tissues by RT-PCR. A specificity of 94% was used to identify marker combinations that offered optimal performance for detection of clinically actionable metastases. The Journal of Molecular Diagnostics 2005 7, 327-336DOI: (10.1016/S1525-1578(10)60561-2) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions