Volume 136, Issue 7, Pages 2149-2158 (June 2009) Sensitive and Specific Detection of Early Gastric Cancer with DNA Methylation Analysis of Gastric Washes  Yoshiyuki Watanabe, Hyun Soo Kim, Ryan J. Castoro, Woonbok Chung, Marcos R.H. Estecio, Kimie Kondo, Yi Guo, Saira S. Ahmed, Minoru Toyota, Fumio Itoh, Ki Tae Suk, Mee–Yon Cho, Lanlan Shen, Jaroslav Jelinek, Jean–Pierre J. Issa  Gastroenterology  Volume 136, Issue 7, Pages 2149-2158 (June 2009) DOI: 10.1053/j.gastro.2009.02.085 Copyright © 2009 AGA Institute Terms and Conditions

Figure 1 Methylation in gastric cancer. Methylation levels of 6 genes (MINT25, RORAa, GDNF, ADAM23, PRDM5, and MLF1) was measured by bisulfite pyrosequencing and normalized by the z score method. (A) Results of individual genes in the test set. (B) Results of individual genes in the validation set. EGC, early gastric cancer; AGC, advanced gastric cancer; GD, gastric dysplasia; NGM, nonneoplastic gastric mucosa; T, tumor tissue in patient with gastric cancer; ADJ, normal adjacent tissue to tumors; W, stomach wash sample in patient with gastric cancer; EN, stomach mucosal tissue in endoscopically normal patient; EW, stomach wash sample in endoscopically normal patient (*P < .05). Gastroenterology 2009 136, 2149-2158DOI: (10.1053/j.gastro.2009.02.085) Copyright © 2009 AGA Institute Terms and Conditions

Figure 2 Correlation of methylation levels between tumor and gastric washes samples. Shown are Spearman correlation coefficients r and P values. Lines show linear regression models. Gastroenterology 2009 136, 2149-2158DOI: (10.1053/j.gastro.2009.02.085) Copyright © 2009 AGA Institute Terms and Conditions

Figure 3 Receiver operating characteristic (ROC) curves for methylation-based detection of gastric cancer. The z score of DNA methylation in test (A) and validation (B) sets were plotted with the use of the top 4 candidate genes in each (test set: MINT25, RORAa, GDNF, ADAM23; validation set: MINT25, ADAM23, PRDM5, GDNF). Gastroenterology 2009 136, 2149-2158DOI: (10.1053/j.gastro.2009.02.085) Copyright © 2009 AGA Institute Terms and Conditions