SCIENCE VOL 301 26 SEPTEMBER 2003 Nanoparticle-Based Bio–Bar Codes for the Ultrasensitive Detection of Proteins Jwa-Min Nam,* C. Shad Thaxton,* Chad A.

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Presentation transcript:

SCIENCE VOL 301 26 SEPTEMBER 2003 Nanoparticle-Based Bio–Bar Codes for the Ultrasensitive Detection of Proteins Jwa-Min Nam,* C. Shad Thaxton,* Chad A. Mirkin Qing Han 20.309 Presentation 10.14.08

Background PCR Immuno-PCR ELISA Sensitivity~pM

Drawbacks of current immuno-PCR A low ratio of DNA to detection Ab Slow target-binding kinetics Link the Ab and DNA markers PCR requirements

The bio-bar-code assay method -----Probe designand preparation. Detection Ab Capture Ab 1 um

PSA detection and bar-code DNA amplification and identification

Scanometric detection of PSA-specific bar-code DNA

Bio–bar-code method offers several advantages Homogeneous target-binding portion High ratio of PCR-amplifiable DNA to Ab No need for complicated conjugation chemistry Low background signal potential for the simultaneous detection of many analytes in one solution

Questions?