ANOVA Within Strain P-values

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ANOVA Within Strain P-values WILD-TYPE DCIN5 DGLN3 DHAP4 DSWI4 p < 0.05 2377 (38.41%) 1995 (32.23%) 1856 (30.00%) 2387 (38.57%) 2583 (41.74%) p < 0.01 1531 (24.74%) 1157 (18.69%) 1007 (16.27%) 1489 (24.06%) 1679 (27.13%) p < 0.001 850 (13.73%) 566 (9.15%) 398 (6.43%) 679 (10.97%) 869 (14.04%) p < 0.0001 449 (7.25%) 280 (4.52%) 121 (1.96%) 240 (3.88%) 446 (7.21%) B&H p < 0.05 1673 (31.88%) 1117 (18.05%) 889 (14.36%) 1615 (20.09%) 1855 (29.97) Bonferroni p < 0.05 226 (3.65%) 109 (1.76%) 20 (0.32%) 61 (0.99%) 179 (2.89%)

Cold Shock Recovery t test t15 t30 t60 t90 t120 582 (9.4%) 865(14.0%) Average Log Fold Change > 0.25 and p < 0.05 582 (9.4%) 865(14.0%) 933(15.1%) 439(7.1%) 230(3.7%) Average Log Fold Change < -0.25 and 486(7.9%) 717(11.6%) 867(14.0%) 309(5.0%) 247(4.0%) Total 1075(17.4%) 1587(25.6%) 1814(29.3%) 749(12.1%) 509(8.2%) Total B & H 0(0%) 85(1.4%) 1(0.016%) Total Bonferroni

Cold Shock Recovery t test t15 t30 t60 t90 T120 WILD TYPE 1075(17.4%) 1587(25.6%) 1814(29.3%) 749(12.1%) 509(8.2%) DCIN5 1393 (22.5%) 756(12.2%) 1250(30.2%) 634(10.2%) 351(5.6%) DGLN3 1027(16.6%) 1622(26.2%) 628(10.1%) 558(9.0%) 403(6.5%) DHAP4 1197(19.3%) 1772(28.6%) 2006(32.4%) 234(3.7%) 515(8.3%) DSWI4 N/A 1497(24.2%) 1757(28.4%) 705(11.4%)

Between Strain ANOVA Comparison Results B&H p <0.05 wt vs. dCIN5 563 4 wt vs. dGLN3 720 36 wt vs. dHAP4 640 23 wt vs. dHMO1 556 5 wt vs. ZAP1 553 31 wt vs. S. par 1498 703

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