Lab 3 Staining.

Slides:

Advertisements

Similar presentations

Lab 6. Gram staining was originally developed in 1844 by Christian Gram Because most bacteria have one of these two types of cell walls, we can use this.

Advertisements

381 PHT Lab #2.

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case M I C.

BACTERIAL STAINING.

BACTERIAL STAINING.

226 PHT Lab#2 Staining techniques

Differential Stains (Gram stain & Acid Fast Stain) Abdelraheem BA

The Size of It All Types of Microscopes. The Size of It All Remember that 1 inch = 2.54 cm and that 1 meter contains micrometers (µm) or

Third lecture for microbiology manual

CHAPTER 3 OBSERVING MICROORGANISMS THROUGH A MICROSCOPE

S TAINING M ICROORGANISMS An overview of staining.

The Gram Stain In the late 1800’s, Christian Gram observed that some genera of bacteria retained a dye-Iodine complex when rinsed with alcohol, while.

226pht Lab#2 Gram staining.

MICROBIOLOGY – ALCAMO LECTURE: SPECIMEN PREPARATION AND STAINING.

Microscopes and staining Procedures

MICROBIOLOGY – ALCAMO LECTURE: SPECIMEN PREPARATION AND STAINING.

Observing Microorganisms Through a Microscope

CHAPTER 3 OBSERVING MICROORGANISMS THROUGH A MICROSCOPE Dr. Reitano SUFFOLK COUNTY COMMUNITY COLLEGE.

StainsStains. Cheek Cells Without Stain Cheek Cells with Simple Stain (Methylene Blue)

Gram staining. Types of staining Simple –Bacteria stained to contrast environment Differential –Bacteria differing chemically react differently to staining.

Experiment 2 Gram Stain and Acid-fast Stain.  Staining - simple stain (only one dye) - differential stain (more than one dye) G+G+ G-G- Gram stain Acid-fast.

Staining Lab 3 and 4 Notes and Pictures. Smears Air dry first to prevent lysis (boiling) Heat Fixing –Kill –Stops autolysis –Adherence to slide.

PREPARATION OF A SMEAR GRAM STAIN ACID FAST STAIN

Lab # 3 Gram and Acid Fast stain Medgar Evers College Biology 261 Prof. Santos.

Biology 11 Microbiology Gram staining. What is Gram Staining? Basic classification of bacteria that is based on the cell wall structure. There are 2 main.

Two -methods are generally used, (wet method) and (dry and fix method (. for Studying Microbes with a Compound Microscope Wet Method. There are two primary.

TOPICS Staining and stain Staining and stain Types of staining techniques Types of staining techniques Gram Staining - Principles Gram Staining - Principles.

THE GRAM STAIN. The gram stain is the most widely used staining procedure in bacteriology It is called a differential stain it differentiates between.

StainsStains. Negative Stain Cheek Cells Without Stain.

Observing Microorganisms through a Microscope

MICROBIOLOGIA GENERALE

Observing Microorganisms through a Microscope Dr. Bhavesh Patel Principal V.P. and R.P.T.P. Science College Vallabh Vidyanagar –

1 Chapter Microscopy. 2 Light Microscopes – uses light passed through a specimen Types include:  Brightfield  Darkfield  Phase-Contrast  Differential.

Observation of bacteria using staining procedures

Gram Staining. Aseptic Technique Sterilize Everything – Use bunsen burner or Bacti-Cinerator.

Bell Ringer Identify the two pictures below with Gram reaction, morphology, and arrangement.

Gram Stain © 2013 Project Lead The Way, Inc.Principles of Biomedical Science.

CHAPTER 3 OBSERVING MICROORGANISMS THROUGH A MICROSCOPE

Differential staining

LECTURE: SPECIMEN PREPARATION AND STAINING

GRAM and ACID FAST STAINS

بسم الله الرحمن الرحيم 140 Micro Lab 7: Gram stain.

Principles of Laboratory Diagnosis of Infectious Diseases

Lecturer Ishraq Hasan Elewi.

Gram Staining: Principle, Procedure and Results MSc. Sarah Ahmed

Gram’s stain Acid fast stain Spore stain Hanging drop technique

Bacterial Cell Walls.

Biology and Biotechnology department

PRACTICAL GRAM STAINING

THE GRAM STAIN.

The Study of Microbial Structure: Microscopy and Specimen Preparation

Why we should be Stain Bacteria

Practical 2 Gram staining.

Observing Microorganisms Through a Microscope

Cocci. Cocci Bacilli Spirochetes Staphylococcus Arrangement.

Lab 3 streptococci.

Observing Microorganisms through a Microscope

Observing Microorganisms Through a Microscope

Practical No. 5 Staining Department of Microbiology

Observing Microorganisms Through a Microscope

Medical Microbiology Lab. 3 By Assistant lecturer Zainab farooq shafeeq.

Types of microbiologic stains

Presentation transcript:

Lab 3 Staining

Staining Bacteria are colorless for the most part. Staining makes bacteria more easily visible. This means that the structures have to be contrasted from their environment so that they can be seen easily. Stain is a dye used to color the living or dead organelles.

Staining Simple staining : only one dye . Ex. Simple stain methylene blue Differential staining: more than one dye . Differentiation among bacteria is possible Ex. Gram stain Special staining: stain Special structures Ex. Capsule stain , spore stain.

Simple stain Use only one dye Ex. Methylene blue or safranin

Gram stain Hans Christian Gram , Danish scientist Classify Bacteria into Gram negative & Gram positive . Thin cell wall Thick cell wall

4 dyes 1- primary stain: ( crystal violet ) 1 minute 2- mordant : ( iodine ) 1 minute 3- decolorizer : ( alcohol or acetone ) 1-2 seconds 4- counterstain : ( safranin ) 1 minute

Smear preparation

common bacterial arrangement

Gram Positive Cocci

Nontypical Cell Walls Several bacterial groups lack the cell wall structure of gram-positive or gram- negative bacteria, and some bacteria have no cell wall at all. Mycobacterium and Nocardia thick, waxy nature cell wall archaea (polysaccharides cell wall) Mycoplasmas ( Does not have cell wall)

Cellular morphology and arrangement (Draw a detailed sketch) Gram Stain Observation and Interpretations Record your observation in the chart below: Organism or source Cellular morphology and arrangement (Draw a detailed sketch) Color Gram reaction (+/-)