Dr. Souki MICRO 200 Cerritos College

Slides:

Advertisements

Similar presentations

Ex. 5: Acid-Fast and Endospore Stains

Advertisements

Plate 14 Staining Bacteria.

Lab Protocol: Gram Staining Yogurt Sample or Milk (raw or pasteurized)

Purpose: Use procedure to identify if bacteria is gram positive or gram negative. This would help someone know how to treat an infection. Prelab: 1. Why.

BACTERIAL STAINING.

COLONY MORPHOLOGY ON AGAR PLATE CULTURES

Observation of bacteria using staining procedures Simple staining Gram staining.

GRAM STAIN PROCEDURE 1. Gather all equipment and supplies Gram stain

Do Now 1.Discuss the primary function of a microscope. 2.Define resolution and justify why electron microscopes are the best tools to use when resolving.

Gram Staining Method Biology 2 Mr. Beyer. Individual Supplies Microscope Slide Clothes Pin Inoculating Loop or Wood Splint Paper Towels.

Microscopy Compound light microscope is composed of: 1- stand 2- stage 3- substage ( condenser, diaphragm) 4- body tube (carrying lens system) 5- light.

Use and Care of Microscope, Aseptic Technique, Smears, Simple Stains

Classification of bacteria

CHAPTER 3 Observing Organisms Through a Microscope Units of Measurements Microscopy: The Instruments Preparation of Specimens.

أ. شروق الشهراني بسم الله الرحمن الرحيم 140 MICRO LAB 6: SIMPLE STAIN + N EGATIVE S TAIN.

MICROSCOPES. Microscopes Microscope : an instruments used to examine very small objects (Specimens) in microbiology. Functions : To allow us to study.

Bacterial Staining General Discussion. Stains All dyes are salts –Ionize Cationic Anionic Techniques –Single dyes –Multiple dyes Use.

S TAINING M ICROORGANISMS An overview of staining.

CHAPTER 3 Observing Organisms Through a Microscope Units of Measurements Microscopy: The Instruments Preparation of Specimens.

One way to classify bacteria is by examining its morphology. Bacteria appear in many different shapes. We will examine the three basic shapes but note.

Gram Staining Microbiology How to Make a Smear  1. Label the frosted side of your slide with your initials, the name of the organism, and the date.

The Gram Stain In the late 1800’s, Christian Gram observed that some genera of bacteria retained a dye-Iodine complex when rinsed with alcohol, while.

Lab Exercise 4: Simple Staining and Bacterial Cell Morphology.

Microbial Ecology – From Isolation to Identification

Simple Staining and Bacterial Cell Morphology

Simple stain Rana A.Abo-Hajjras. Simple stain The simple stain can be used to determine cell shape, size, and arrangement. True to its name, the simple.

Aseptic techniques, smear preparation and simple staining Lab # 2 Bio 261, Microbiology Prof. Santos.

What is a Stain A stain is a substance that adheres to a cell, giving the cell color. The presence of color gives the cells significant contrast so are.

MICROBIOLOGY – ALCAMO LECTURE: SPECIMEN PREPARATION AND STAINING.

Lab #5. Review for Practical #1 Colony morphology (pg. 19)

Microbiology Professor Sidelsky 2007

Focusing the microscope simple staining

BIOLOGY & BIOTECHNOLODY DEPARTMENT.  In our laboratory, bacterial morphology (form and structure) may be examined in two ways: 1.By observing living.

MICROBIOLOGY LAB 5 BIDISHA PAUL 261-BH.

Lab 3 Goals and Objectives :

Class Four Microbiology Quiz  Quiz Number One – Open Response and Identification  Microscope  Aseptic techniques  Culture transfer techniques.

PREPARATION OF A SMEAR GRAM STAIN ACID FAST STAIN

Acid-Fast and Endospore Stains Objectives: Explain the rationale and procedure for the acid-fast and endospore stains Perform and interpret the acid fast.

أ. شروق الشهراني بسم الله الرحمن الرحيم 140 MICRO LAB 6: S IMPLE S TAIN + N EGATIVE S TAIN.

Gram staining Techniques. Some history Bacteria are translucent Bacteria are translucent Staining make them visible under microscope Staining make them.

Safety Notes Bunsen burners> open flame

Two -methods are generally used, (wet method) and (dry and fix method (. for Studying Microbes with a Compound Microscope Wet Method. There are two primary.

Simple Stains & Gram stain

Microbial staining Lab 6 Abeer Saati. Microbial staining Since bacterial organisms are so minute, it is impossible to view the organisms without compound.

Gram Staining Method Mr. Beyer. Individual Supplies Microscope Slide Clothes Pin Innoculating Loop Paper Towels.

Copyright © 2010 Pearson Education, Inc. Bell Ringer  A new chemical was synthesized that inhibits the Golgi body from functioning. How would this affect.

CHAPTER 3 OBSERVING MICROORGANISMS THROUGH A MICROSCOPE

PRACTICAL MICROBIOLOGY

LECTURE: SPECIMEN PREPARATION AND STAINING

Bacterial Morphology Bacteria: are unicellular free living organisms without chlorophil having doth DNAand RNA.

GRAM and ACID FAST STAINS

PRACTICAL GRAM STAINING

Principles of Laboratory Diagnosis of Infectious Diseases

PRACTICAL GRAM STAINING

Faculty of Medicine and Health Sciences

Pure Culture Techniques

PRACTICAL GRAM STAINING

Practical 2 Gram staining.

Week 4W Review cultures: Exp 2B Lab Two & Exp 3 Lab Two

The process of adding a dye to a bacterial culture

BIOLOGY & BIOTECHNOLODY DEPARTMENT

Practical No. 5 Staining Department of Microbiology

140mic General Microbiology

Simple Staining and Bacterial Cell Morphology

SHAPE OF THE BACTERIA.

Bacterial Structure (Lab 3)

COLONY MORPHOLOGY ON AGAR PLATE CULTURES

Presentation transcript:

Dr. Souki MICRO 200 Cerritos College 5-Simple Stains Dr. Souki MICRO 200 Cerritos College

Purpose To determine cell morphology and arrangement using the simple stain method.

What is a stain? Stains contain a solvent (water or ethanol) and colored molecule (chromogen). Chromogen Auxochrome: charged part of the chromogen Chromophore: color auxochrome chromophore Auxochrome does not absorb light, rather it intensifies absorption of light by its conjugate chromophore. Intensifies the color of the chromophore.

What is a basic stain? Basic stains contain a positively charged auxochrome. Examples of basic stains: Crystal violet Methylene blue Safranin Malachite green Crystal Violet Methylene Blue Malachite Green Safranin

How is a bacterial smear prepared? Flame an inoculating loop. Cool the loop. Place a small drop of water onto a clean slide using an inoculating loop.

How is a bacterial smear prepared? 3. Flame and cool the inoculating loop. 4. Use the loop to grab bacteria from a slant or broth culture. 5. Mix the bacteria into an emulsion onto the slide using the loop. 6. Let it dry.

How is the smear fixed to the slide? 7. Heat fix the smear by passing the smear through the flame 3 times at the inner cone of the flame. Heat fix because: Kills the cells (denatures proteins. Denatured proteins fixes the cells to the slide.

How is the heat-fixed sample stained? Prepare a heat fixed bacterial smear. Flood slide with crystal violet for 1 min. Rinse with water gently. Blot dry with Kimwipe. View under oil (1000X)

What shapes do bacteria form?

What arrangements do bacteria form?

Cell arrangements

Answer the following questions in your notebook. Describe what physical differences distinguish the bacteria? How will you tell them apart? Explain the difference between streptococci and streptobacilli. Why is flaming the inoculation loop important? What part of the flame is best for flaming the inoculation loop. Why? Explain why working near the flame is important when opening culture tubes. Explain what is a simple stain and how it is used. Explain the proper procedure for cleaning an oil immersion lens after use. Why should you NOT use Kimwipes to clean microscope lenses? What should you use?