Figure 4 Ex vivo synovial tissue culture viability

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Figure 4 Ex vivo synovial tissue culture viability Figure 4 | Ex vivo synovial tissue culture viability. To establish ex vivo rheumatoid synovial biopsies, whole synovial tissue can be transferred directly from the biopsy forceps to culture medium. Synovial tissue can be maintained in culture for up to 72 h and remains viable during this time. a | Representative photomicrographs of synovial tissue, after 72 h in culture, stained with fluorescent calcein green indicating viable cell nuclei (white arrows). b | Viable blood vessels in cultured synovial tissue (white arrow). c,d | Representative photomicrographs of synovial tissue, after 72 h in culture, embedded in optimal cutting temperature (OCT) compound and stained with haematoxylin and eosin to demonstrate the intact structural morphology of the synovial tissue: intact lining layer, sublining layer and blood vessels (black arrows). Original magnifications ×10. Orr, C. et al. (2017) Synovial tissue research: a state‑of‑the-art review Nat. Rev. Rheumatol. doi:10.1038/nrrheum.2017.115