Today: Ionic Equilibria Wrap- up Recombinant DNA and Biotechnology

Next Up: DNA Technology What’s interesting, exciting, frightening? Capture your thoughts: Question 1

DNA Tools: Restriction Enzymes What do Restriction Enzymes do? Where do they cut (and why)??

DNA Tools: Restriction Enzymes

DNA Tools: Gel Electorphoresis You Try! Question 2

DNA Tools: Southern Blots You Try! Question 3

DNA Tools: Western and Northern Blots What can a Northern or Western Blot tell us that a Southern can’t?? You Try: Question 4

DNA Tools: Fingerprints DNA Fingerprints can be made from highly variable regions (polymorphisms). Two are commonly used: Single Nucleotide Polymorphisms (SNPs) and Short Tandem Repeats (STRs)

DNA Tools: Fingerprints You Try! Question 5

DNA Tools: Barcodes?!?

Building Recombinant DNA

DNA Tools: Vectors Good Vectors: Replicate Independently Provide Recognition Sequences for Restriction Enzymes Include a Reporter Gene Are Small

Where do you get DNA to Clone?? DNA (Gene) Libraries? Are all these clones useful to you?

How Could You Get a Library of Expressed, Edited Genes?!?

DNA Tools: Knock-Outs

DNA Tools: Antisense RNA

DNA Tools: Micro-arrays Let’s watch! http://www.bio.davidson.edu/Courses/genomics/chip/chip.html

Applications: Medically Useful Proteins

Applications: Medically Useful Proteins

Applications: Pharming Webster and Pete; Photo BBC

Applications: Agriculture

Applications: Creating Life?! Let’s Watch! http://www.ted.com/index.php/talks/view/id/227 Capture your thoughts! Question 6