HMGB1 is required for the in vivo adjuvant activity of necrotic cells.

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HMGB1 is required for the in vivo adjuvant activity of necrotic cells. HMGB1 is required for the in vivo adjuvant activity of necrotic cells. (A) HMGB1 is expressed in the nuclei of wild‐type (+/+, bottom) fibroblasts, whereas Hmgb1−/− fibroblasts (top) do not express it. Nuclei are revealed by staining with DAPI, and HMGB1 expression by immunohistochemistry. (B) HMGB1 in the supernatant of necrotic wild‐type (+/+) or Hmgb1 knockout (−/−) fibroblasts was revealed by immunoblotting. (C) The expression of HLA‐DR, CD40, CD83, CD80 and CD86 was assessed by flow cytometry on untreated immature DCs (a) or DCs treated with the supernatants of wild‐type necrotic fibroblasts (F/T +/+; b) or their −/− counterparts (c). Only DCs treated with supernatants of necrotic Hmgb1+/+ fibroblasts had significantly higher expression of the markers (P<0.005). (D) The expression of HLA‐DR, CD80 and CD86 was assessed on immature DCs, either untreated or treated with the supernatants of necrotic Hmgb1+/+ fibroblasts (F/T +/+ sup), insoluble fractions of necrotic Hmgb1+/+ fibroblasts (F/T +/+ pellet), supernatants of necrotic Hmgb1−/− fibroblasts (F/T −/− sup) and insoluble fractions of necrotic Hmgb1−/− fibroblasts (F/T −/− pellet). Results are expressed as a relative increase (y‐axis) in surface expression over untreated DCs. DCs treated with pellets or F/T −/− sup had significantly lower expression of the different markers (*P<0.01). Experiments were repeated at least three times with DCs from different donors. (E) The production of TNF‐α by immature DCs treated with F/T −/− sup or F/T −/− sup reconstituted with purified recombinant HMGB1 was assessed in the cell culture supernatants. (F) The development of lymphoma was evaluated in C57BL/6 mice vaccinated with PBS (saline), apoptotic RMA cells or apoptotic RMA cells in the presence of the supernatants of wild‐type necrotic fibroblasts (F/T +/+) or from their Hmgb1−/− counterparts (F/T −/−). The results shown depict the fraction of tumour‐free mice (y‐axis) at different times after injection of living RMA lymphoma cells (x‐axis). Protected mice were re‐challenged on day 80 (arrow). (G) The results shown depict the mean diameter of the growing tumour (y‐axis) at different times after administration of living RMA cells (x‐axis). Fisher's exact test results for protection and tumour growth were **P<0.005 and ***P<0.001, respectively. Patrizia Rovere‐Querini et al. EMBO Rep. 2004;5:825-830 © as stated in the article, figure or figure legend