FIGURE 1 CONSORT flow diagram

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FIGURE 1 CONSORT flow diagram FIGURE 1 CONSORT flow diagram. CONSORT, Consolidated Standards of Reporting Trials. From: A randomized controlled trial of the impact of protein supplementation on leg lean mass and integrated muscle protein synthesis during inactivity and energy restriction in older persons Am J Clin Nutr. 2018;108(5):1060-1068. doi:10.1093/ajcn/nqy193 Am J Clin Nutr | © 2018 American Society for Nutrition.This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

FIGURE 2 Schematic of study design FIGURE 2 Schematic of study design. CP, collagen peptide supplement; DXA, dual-energy X-ray absorptiometry; WP, whey protein supplement. From: A randomized controlled trial of the impact of protein supplementation on leg lean mass and integrated muscle protein synthesis during inactivity and energy restriction in older persons Am J Clin Nutr. 2018;108(5):1060-1068. doi:10.1093/ajcn/nqy193 Am J Clin Nutr | © 2018 American Society for Nutrition.This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

FIGURE 3 Changes in (A) LBM between sequential study phases, and (B) LLM between sequential study phases. The box plot shows the median (line) and mean (+), with the box representing the IQR, and the whiskers representing the maximum and minimum values. Data were analyzed with 2-factor ANOVA with group as a between factor, and repeated measures for phase. WP (n = 16), CP (n = 15). Means that do not share a letter are significantly different within the group, P < 0.05. *Differences between groups at that time point, P < 0.05. P value indicates the interaction of WP and CP at RC. CP, collagen peptide; ER, energy-restricted phase; ER + SR, energy-restricted and step-reduction phase; LBM, lean body mass; LLM, leg lean mass; RC, recovery phase; WP, whey protein. From: A randomized controlled trial of the impact of protein supplementation on leg lean mass and integrated muscle protein synthesis during inactivity and energy restriction in older persons Am J Clin Nutr. 2018;108(5):1060-1068. doi:10.1093/ajcn/nqy193 Am J Clin Nutr | © 2018 American Society for Nutrition.This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

FIGURE 4 Rates of integrated myofibrillar muscle protein synthesis (%/d) during EB, ER, ER + SR, and RC. The box plot shows the median (line) and mean (+), with the box representing the IQR and the whiskers representing the maximum and minimum values. Data were analyzed with 2-factor ANOVA with group as a between factor and repeated measures for phase. WP (n = 16), CP (n = 15). Means that do not share a letter are significantly different within the group, P < 0.05. *Differences between groups at that time point, P < 0.05. P value indicates the interaction of WP and CP at RC. CP, collagen peptide; EB, energy balance; ER, energy restriction; ER + SR, energy restriction and step reduction; RC, recovery; WP, whey protein. From: A randomized controlled trial of the impact of protein supplementation on leg lean mass and integrated muscle protein synthesis during inactivity and energy restriction in older persons Am J Clin Nutr. 2018;108(5):1060-1068. doi:10.1093/ajcn/nqy193 Am J Clin Nutr | © 2018 American Society for Nutrition.This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)